<![CDATA[[陽明校區] 超解析螢光顯微鏡含活細胞培養系統 Zeiss Elyra 7 with Lattice SIM²]]><![CDATA[<div class="ed_model05 clearfix">
<div class="ed_pic_right"><img alt="超解析螢光顯微鏡含活細胞培養系統 Zeiss Elyra 7 with Lattice SIM2" src="/userfiles/ordch/images/20231013110037378.jpg" /></div>
<div class="ed_model01 clearfix">
<ul style="list-style-type: disc;">
<li class="ed_txt"><strong>Super-Resolution Microscope with Lattice SIM ² and Incubation System</strong></li>
<li class="ed_txt"><strong>儀器位置：陽明校區 圖資大樓6樓 617室</strong>
<ul>
<li class="ed_txt">分機 66185</li>
</ul>
</li>
<li class="ed_txt"><strong>負責人：陽明校區影像核心設施專員</strong>
<ul>
<li class="ed_txt">陳珮君小姐 <a href="mailto:pjchen3@nycu.edu.tw" title="pjchen3@nycu.edu.tw">pjchen3@nycu.edu.tw</a><br />
呂詠玉小姐 <a href="mailto:yungyu@nycu.edu.tw" title="yungyu@nycu.edu.tw">yungyu@nycu.edu.tw</a></li>
</ul>
</li>
<li class="ed_txt"><strong>使用資格： 認證制</strong>
<ul>
<li class="ed_txt">須參加原理與儀器介紹課程、有confocal上機經驗，並經專員實機操作教學後，才能申請認證考試。</li>
<li class="ed_txt">須通過認證考試，並於日間時段自行操作5次(含)以上者，才具預約權限。</li>
</ul>
</li>
<li class="ed_txt"><strong>校內使用每小時1300元，校外請見收費標準</strong></li>
<li class="ed_txt"><strong>預約 >>> <a href="http://ircbooking.nycu.edu.tw/IRC/irc_booking/booking_login.aspx" rel="noreferrer noopener" target="_blank" title="儀器預約系統(開啟新視窗)">儀器預約系統</a></strong></li>
<li class="ed_txt"><strong>初次使用請先填寫<a href="https://forms.gle/k1hr3wtmjj8G85am8" rel="noreferrer noopener" target="_blank" title="樣品資訊調查表(開啟新視窗)">樣品資訊調查表</a>，待專員回覆確認時間。</strong>樣品測試：每實驗室同類型樣品限預約一次（測試/教學至多折抵兩小時收費），重複預約須依使用時段收費！</li>
</ul>
</div>
</div>]]>儀器資源中心114-11-13影像核心設施專員分機 66185儀器規格<div class="ed_model01 clearfix">
<div class="ed_txt"><strong>顯微鏡系統(Microscope):</strong></div>
<ul style="list-style-type: disc;">
<li class="ed_txt">全電動倒立式顯微鏡 Axio Observer 7 SP</li>
<li class="ed_txt">具 Piezo xyz-stage</li>
</ul>
<div class="ed_txt"><strong>物鏡(Objective):</strong></div>
<ul>
<li class="ed_txt">10X (Apotome)</li>
<li class="ed_txt">20X (Apotome)</li>
<li class="ed_txt">40X oil (Apotome)</li>
<li class="ed_txt">63X oil (SIM)</li>
<li class="ed_txt">100X oil (TIRF)</li>
</ul>
<div class="ed_txt"><strong>雷射波長(Laser (nm)):</strong></div>
<ul>
<li class="ed_txt">405, 488, 561, 642</li>
</ul>
<div class="ed_txt"><strong>觀察模式(Observation Methods):</strong></div>
<ul>
<li class="ed_txt">Apotome</li>
<li class="ed_txt">Lattice SIM</li>
<li class="ed_txt">TIRF</li>
</ul>
<div class="ed_txt"><strong>螢光濾鏡(Emission Filter):</strong></div>
<ul>
<li class="ed_txt">目鏡觀察：Filter set 77 HE GFP + mRFP + Alexa 633 shift free</li>
<li class="ed_txt">影像擷取：
<ul>
<li class="ed_txt">Position 3: EF LBF 405/488/561/642</li>
<li class="ed_txt">Position 4: BP 420-480 / BP 495-550 / LP 655</li>
<li class="ed_txt">Position 5: EF BP 495-550/BP 570-620</li>
<li class="ed_txt">Position 6: BP 420-480/LP 655</li>
</ul>
</li>
</ul>
<div class="ed_txt"><strong>照相設備(Camera):</strong></div>
<ul>
<li class="ed_txt">sCMOS</li>
</ul>
<div class="ed_txt"><strong>活細胞培養系統(Live-cell Incubation):</strong></div>
<ul>
<li class="ed_txt">全罩式與小型活細胞培養系統，具溫濕度及CO<sub>2</sub>控制模組</li>
<li class="ed_txt">小型活細胞培養系統架設於載物台上，二氧化碳穩定控制濃度範圍0.0%至10.0% （±0.1%），適用於底部為NO.1.5 (0.17mm)玻璃蓋玻片之直徑35mm培養皿與76x26mm標準玻片。</li>
<li class="ed_txt">如需使用活細胞系統，初次預約前請先來電詢問管理員。</li>
<li class="ed_txt">具Definite Focus 2 自動焦距維持系統影像軟體。</li>
</ul>
<div class="ed_txt"><strong>影像軟體(Software):</strong></div>
<ul>
<li class="ed_txt">Zen</li>
<li class="ed_txt">Super-resolution module: SIM, SIM<sup>2</sup> 模組</li>
<li class="ed_txt">Apotome</li>
<li class="ed_txt">備有離線電腦工作站可供影像處理與分析</li>
</ul>
</div>樣品製備建議<div class="ed_model01 clearfix">
<ol>
<li class="ed_txt"><strong>Coverslip</strong>
<ul>
<li class="ed_txt">We recommend using <strong>high-precision cover glasses with<span style="color:#ff0000;"> thickness no. 1.5</span></strong><span style="color:#ff0000;"> </span>to prepare the samples.
<ul>
<li class="ed_txt">Zeiss 474030-9010-000 Deckgläser 1,5H hi 100002240928 QS; Thickness no 1 ½ , high-performance 18 mm x 18 mm, 0.170 +/- 0,005 mm<br />
<img alt="Usage specifications" src="/userfiles/ordch/images/20231012152616164.png" /></li>
<li class="ed_txt">Marienfeld Superior: www.marienfeld-superior.com
<ul>
<li class="ed_txt">18×18 mm (Cat.No. 0107032).22×22 mm (Cat.No. 0107052).</li>
<li class="ed_txt">round with 18 mm diameter (Cat.No. 0117580).</li>
</ul>
</li>
</ul>
</li>
<li class="ed_txt">Fix your cells according to your preferred protocol.</li>
<li class="ed_txt"><strong>Please make sure that the <span style="color:#ff0000;">cover slip is centered on the glass slide</span></strong>, in order to fit in our samples holder.</li>
<li class="ed_txt">For glass-bottom petri dishes and multi-well plates see:
<ul>
<li class="ed_txt"><a href="http://www.willcowells.com/" rel="noreferrer noopener" target="_blank" title="Willco (開啟新視窗)">Willco </a></li>
<li class="ed_txt">Lab-tek (offered through Thermo)</li>
<li class="ed_txt">MatTek (<a href="https://www.mattek.com/products/glass-bottom-dishes/" rel="noreferrer noopener" target="_blank" title="glass bottom dishes(開啟新視窗)">glass bottom dishes</a>)</li>
<li class="ed_txt">ibidi µ-Dish 35 mm, high Glass Bottom (Cat.No: 81158)</li>
</ul>
</li>
</ul>
</li>
<li class="ed_txt"><strong>Mounting Media</strong>
<ul>
<li class="ed_txt">Suggested Hardening media:
<ul>
<li class="ed_txt"><strong>ProLong™ Glass Antifade Mountant P36980 RI 1.52</strong></li>
<li class="ed_txt"><strong>ProLong™ Gold Antifade Mountant P36930 RI 1.46</strong></li>
</ul>
</li>
<li class="ed_txt">As it is a hardening media, you may also notice it can somewhat flatten samples.</li>
<li class="ed_txt">Do not use mounting media that include DAPI.</li>
<li class="ed_txt">Concerning the refractive index (RI) of the mounting medium, <span style="color:#ff0000;">prepare the slides at least 2 days before imaging</span>. Allowing the sample to have sufficient time for curing mounting media and sealant. (2–8°C)</li>
<li class="ed_txt">The following Aqueous(Non-Hardening) Mounting liquid media also works, despite having a lower refractive index. SlowFadeGold /Diamond RI of 1,42. VectaShield H-1000. RI of 1,44</li>
</ul>
</li>
<li class="ed_txt"><strong> Fluorescent Labels for SR-SIM</strong></li>
</ol>
<div class="ed_txt" style="margin-left: 40px;">All common types of organic dyes usually conjugated to antibodies or fluorescent proteins are suitable to be used for SR-SIM. <strong>For multicolor samples the fluorophores should be selected for minimal spectral overlap to avoid crosstalk.</strong></div>
<ul style="margin-left: 40px;">
<li class="ed_txt">405 nm (50 mW),</li>
<li class="ed_txt">488 nm (100 mW),</li>
<li class="ed_txt">561 nm (100 mW),</li>
<li class="ed_txt">642 nm (150 mW)</li>
</ul>
<div class="ed_txt" style="margin-left: 40px;">Note： <span style="color:#ff0000;">Cytosolic or other non-specific fluorescent protein expression (e.g. GFP) will result in staining of extended areas</span>. Since well-defined structures are missing to interfere with the grid pattern, modulation contrast will be low and the final image will lack high resolution information.</div>
</div>實驗應用<img alt="SIM2 " src="/userfiles/ordch/images/20241220170332746.png" /><br />
<img alt="SIM Apotome" src="/userfiles/ordch/images/20241220170332452.png" />預約規則<div class="ed_model01 clearfix">
<div class="ed_txt"><strong>＊本機採認證制＊</strong><br />
<strong>須參加原理與儀器介紹課程、有confocal上機經驗，並經專員實機操作教學後，才能申請認證考試</strong>，認證考試通過後須於日間時段自行操作5次(含)以上者，才具預約與門禁權限。兩星期內每人最多可預約4次，每時段最多4小時。</div>
<ul>
<li class="ed_txt">預約規定：
<ul>
<li class="ed_txt"><strong>初次使用請先填寫<a href="https://forms.gle/k1hr3wtmjj8G85am8" rel="noreferrer noopener" target="_blank" title="樣品資訊調查表(開啟新視窗)">樣品資訊調查表</a>，待專員回覆確認時間。</strong></li>
<li class="ed_txt"><strong>樣品測試：每實驗室同類型樣品限預約一次（測試/教學至多折抵兩小時收費），重複預約須依使用時段收費！</strong></li>
<li class="ed_txt">使用前請至儀器中心「<a href="http://ircbooking.nycu.edu.tw/IRC/irc_booking/booking_login.aspx" rel="noreferrer noopener" target="_blank" title="儀器預約系統(開啟新視窗)">儀器預約系統</a>」進行預約，預約者須為實際使用者（不得使用他人證件進出）。</li>
<li class="ed_txt">儀器使用完畢須確實清潔、關機，並詳實填寫登記本。起始時間為預約時間，終止時間則視實際結束使用時間為準。</li>
<li class="ed_txt">不可延遲上機時間或佔用他人時段。</li>
<li class="ed_txt">最遲請於前一個工作天16:30前確定，逾時變更視同違規，不予接受。</li>
<li class="ed_txt">不可當日取消預約，預約時間逾時半個小時不到者亦視同放棄。</li>
</ul>
</li>
</ul>
</div>開放時間<ul>
<li class="ed_txt">週一至週五
<ul>
<li class="ed_txt">全天 限認證操作者登記使用</li>
</ul>
</li>
<li class="ed_txt">週末
<ul>
<li class="ed_txt">暫不開放</li>
</ul>
</li>
</ul>收費標準<div class="table01">
<table class="ed_table caption-top" style="width:800px;" summary="儀器收費">
<caption>儀器收費 (NTD per hour)</caption>
<tbody>
<tr>
<th>特殊光學顯微鏡</th>
<th>超解析影像系統含活細胞培養裝置</th>
</tr>
<tr>
<td class="ed_pc_center" data-th="螢光顯微鏡">機型</td>
<td data-th="全玻片掃描系統" style="text-align: center;">(Z) Elyra 7</td>
</tr>
<tr>
<td class="ed_pc_center" data-th="螢光顯微鏡">校內師生收費</td>
<td data-th="全玻片掃描系統" style="text-align: center;">1300</td>
</tr>
<tr>
<td class="ed_pc_center" data-th="螢光顯微鏡">建教合作收費</td>
<td data-th="全玻片掃描系統" style="text-align: center;">1950</td>
</tr>
<tr>
<td class="ed_pc_center" data-th="螢光顯微鏡">其他單位收費</td>
<td data-th="全玻片掃描系統" style="text-align: center;">2600</td>
</tr>
<tr>
<td class="ed_pc_center" data-th="螢光顯微鏡">備註</td>
<td data-th="全玻片掃描系統">
<ol>
<li>廠牌縮寫：(Z) Zeiss</li>
<li>預約次數：兩周內每人每台儀器可預約四次，每時段最多四小時，可連續預約。</li>
<li>除了中心舉辦之影像核心設施教育訓練外，預約教學與認證考試須自備樣品（實驗數據可帶走），並比照一般上機使用收費。</li>
<li><strong>上表為每小時使用費，收費計算以每半小時為單位。</strong></li>
<li>樣品測試：每實驗室同類型樣品限預約一次（<strong>測試/教學至多折抵兩小時收費</strong>），重複預約須依使用時段收費！</li>
</ol>
</td>
</tr>
</tbody>
</table>
</div>
<div class="ed_model01 clearfix">
<div class="ed_txt"><br />
若具以下資格者，收費比照【本校師生】：</div>
<ol>
<li class="ed_txt">分校 </li>
<li class="ed_txt">合聘教師 </li>
<li class="ed_txt">附設醫院 </li>
<li class="ed_txt">與本校有計畫合作且計畫屬本校管控</li>
</ol>
<div class="ed_txt">若具以下資格者，收費比照【建教合作單位】：</div>
<ol>
<li class="ed_txt">兼任教師 </li>
<li class="ed_txt">教學醫院（榮民總醫院、臺北市立聯合醫院、亞東醫院、振興醫院等）</li>
<li class="ed_txt">台聯大系統</li>
<li class="ed_txt">產學運籌中心進駐廠商</li>
</ol>
<div class="ed_txt"><strong>儀器以每半小時為收費單位</strong>，每奇數月月底統計開單，最遲請於兩週內，攜帶繳費單至行政大樓出納組繳費，領取收據，逾期次數達（含）兩次者，將停止對該實驗室提供服務。</div>
</div>注意事項<div class="ed_model01 clearfix">
<ol>
<li class="ed_txt"><strong>本機禁用隨身碟</strong>，可用 FTP 傳輸檔案或請自行攜帶空白光碟片燒錄資料。由於電腦硬碟空間有限，為有利儀器之正常操作，管理人員將不定時刪除資料，本中心不負責資料保管，敬請見諒。</li>
<li class="ed_txt">未經本中心管理員同意，<strong>不得擅自於本中心公用儀器上加裝或拆換任何零件、更改系統設定及安裝軟體。若因實驗需要進行任何測試，須先徵求管理員同意</strong>，並由原廠工程師陪同，在不傷害正常儀器操作之情況下進行測試，通過測試後方可在本中心管理員許可情況下，由管理員認可之人員操作使用。</li>
<li class="ed_txt">依管理員判定違規者第一次警告、第二次取消使用權一個月、第三次永久取消使用權；如違反上述規範，以致儀器損壞者，需照價賠償以利修復儀器，並停止使用權一個月，再違規者則永遠禁止使用。</li>
</ol>
</div>相關檔案<div class="ed_model17 clearfix ed_list">
<ul>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">儀器介紹：EN_product-info_Elyra-7</span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231012153808178.pdf" rel="noreferrer noopener" target="_blank" title="EN_product-info_Elyra-7(pdf)(開啟新視窗)"><img alt="EN_product-info_Elyra-7" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">儀器操作手冊：Manual (20230314 version)</span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231012153750207.pdf" rel="noreferrer noopener" target="_blank" title="Manual (20230314 version)(pdf)(開啟新視窗)"><img alt="Manual (20230314 version)" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">認證操作申請單：Certification form </span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231012153750207.pdf"><img alt="Manual (20230314 version)" src="/userfiles/file/pdf.png" /></a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">原理與儀器介紹：The New Era of Super-resolution Microscopy, Elyra 7</span> </span>
<ul class="d-flex">
<li><a href="https://youtu.be/g2L9bJmY-E4?si=llHEoYBUW6c38SPf" rel="noreferrer noopener" target="_blank" title="The New Era of Super-resolution Microscopy, Elyra 7(開啟新視窗)"><img alt="The New Era of Super-resolution Microscopy, Elyra 7" src="/userfiles/file/link.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;">ZEISS Knowledge Base－線上 Manual 資料庫</span>
<ul class="d-flex">
<li><a href="https://knowledge.zeiss.com/rms" rel="noreferrer noopener" target="_blank" title="ZEISS Knowledge Base(開啟新視窗)"><img alt="ZEISS Knowledge Base" src="/userfiles/file/link.png" /> </a></li>
</ul>
</li>
</ul>
</div>https://ord.nycu.edu.tw/ord/ch/app/machine/image?module=machine&detailNo=1161931470361595904&init=Y超解析顯微鏡含活細胞培養系統-Zeiss Elyra 7https://www.youtube.com/embed/g2L9bJmY-E4youtubehttps://ord.nycu.edu.tw/ord/ch/app/artwebsite/view?module=artwebsite&id=1752&serno=4d94c595-2732-408d-9e9f-ee9f12d73bcd陽明校區儀器列表https://ord.nycu.edu.tw/ord/ch/app/artwebsite/view?module=artwebsite&id=1753&serno=2289d2eb-4b49-4130-a158-72c747082ace陽明校區-影像核心設施https://ord.nycu.edu.tw/ord/ch/app/folder/1759光學顯微鏡類