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Basic Service-Chemistry

exchangealt 中文
  • Update Date：2024-02-06
  • Units：Instrumentation Resource Center
High Resolution Fluorescence Imaging Flow Analyzer
HIGH RESOLUTION FLUORESCENCE IMAGING FLOW ANALYZER
  • High resolution fluorescence imaging flow analyzer (ImageStreamX Mark II)
    Brand model: Taiwan Merck Co., Ltd. ISX500
  • Instrument expert: Prof. Hsu, Hsin-Yun
    ext 56556
  • Instrument consulting and operation services: Ms. Hsieh, Pei-Ying
    ext 56544
    Email plc5sk@gmail.com
  • Instrument location: Room 216, Science Hall II, Guanfu Campus
Instrument brand, model, purchase period
Brand: Taiwan Merck Co., Ltd.
Model: ISX500
Purchase period: 2016/10

Important Specifications
  1. Contains 5 lasers:
    • Blue Laser 488 nm 200 mW
    • Red Laser 642 nm 150 mW
    • Violet laser 405 nm 120 mW
    • UV laser 375 nm 70 mW
    • Green laser 561 nm 200 mW
  2. Can detect at least 12 different signal parameters (Channels).
  3. Equipped with 3 different objective lenses: 20X, 40X, 60X.
  4. It can capture the fluorescence signal of flow cytometer and the image information of fluorescence microscope at the same time.
  5. The liquid flow system is the basis for single cell fluorescence signal detection and image capture, rather than the camera system, which captures a large range of photos and uses software to simulate the flow cytometer.
  6. Fluorescence sensitivity is less than or equal to 5 MESF.
  7. The sample volume should be less than or equal to 20ul.
  8. With absolute cell count function.
  9. Minimum detectable cell size, less than or equal to: 0.3 um.
  10. Maximum detectable cell size, greater than or equal to: 60 um.
  11. Loading vessels containing 1.5 ml as well as 0.5 ml microcentrifuge tubes can be used.
  12. The fastest sample loading speed is at least: 5000 events/Sec.
  13. The detection range (Dynamic Range) is greater than or equal to 4.5 Log
service items
  1. Multicolor Fluorescence Analysis
  2. Biomolecular Analysis
  3. Novel Fluorescent Molecular Analysis

Instrument operation appointment
please go to National Science Council Basic Research Core Facility Reservation Service Management System or call an operating technician
Opening hours
Monday to Friday 9:30~17:00; commissioned services are accepted.

System open level
  1. Normal working hours: D
  2. Night time and holidays: closed
System open level description
Management method and use method
  1. The use of this equipment can only be used after making an appointment and confirmation with the instrument operating technician one week ago. It cannot be cancelled within three days before the appointment period. If the appointment is not cancelled three days ago, the start-up fee will still be charged. If you have any questions, please feel free to call or e-mail to discuss.
  2. Please download the ImageStream Experimental Evaluation Worksheet (Appendix 1) one week before using the instrument, complete it and return it to the instrument operator, and refer to the Sample Preparation Guide (Appendix 2).
  3. In order to facilitate the use of units by other schools, users of other schools can register to use the opening hours during the day.
  4. In order to avoid clogging the pipeline, the sample (and the control group) should be filtered with an appropriate pore size filter first, and please estimate the sample size in advance. The sample concentration is recommended to be at least 1×10.4 cells/50ml (2×107 cells/ml) to avoid lengthy occupancy of the instrument.
  5. Access to experimental data is limited to (VCD/DVD) discs and cannot be stored in any other way. Please bring your own discs, if not, a burning fee of RMB 100 will be charged for each disc
  6. In addition to the sheath fluid, you need to prepare the required samples and experimental reagents (antibodies, fluorescent dyes, etc.), control groups and buffers for suspended samples, etc. Please note that isotopes and infectious reagents and samples are prohibited. its use.
  7. Thank you for using the service of this instrument. If our work is really helpful to your work, please consider listing us as a co-author or an acknowledgement, which will be of great help to the long-term operation and maintenance of this instrument, application for funding, and performance appraisal.
  8. Welcome to cooperate in the development of new technologies and applications of this instrument. If you are interested, please contact the teacher in charge of the instrument.
Charges
Billing item Service Content Plan reservation fee Unplanned reservation fee
Warm-up fee Instrument startup and calibration NT$300  NT$1,000
On-board usage fee Delegate operation NT$350/hour NT$1,500/hour
Data analysis Image processing and data storage NT$500/hour NT$500/hour
  • ImageStream-Experimental-Evaluation-Worksheet-Appendix 1
  • Sample-Preparation-Guide-Appendix II
List of Instruments Basic Service-Chemistry
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