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Confocal Microscopes

exchangealt 中文
  • Update Date：2024-08-22
  • Units：Instrumentation Resource Center
[YangMing] Inverted Laser Scanning Confocal Microscope - Zeiss LSM700
Laser Scanning Conjugate Focus Microscopy Zeiss LSM700
  • Inverted Laser Scanning Confocal Microscope
  • Instrument Location: Room 640, 6th Floor, Library, Information & Research Building
  • Person in charge: YM Campus Imaging Core Facility
  • Term of use: Certified Operation 
    • Only those who have passed the certification test after the instruction and have operated the device more than 5 times during office hours (08:30-16:30) will be authorized to make a reservation.
  • Charges: NT$500 per hour for NYCU users. Please refer to the charging standard for off-campus use.
  • Reservation >>> Instrument Reservation System
  • ［LSM700 Upgrade Announcement］
    Attention: As the LSM700 parts have been discontinued, to avoid months of temporary downtime during future failures, the center will be upgrading the equipment from late September to early October (one week in advance), so please plan and arrange your experiments in advance to minimize the impact of downtime.  
    After the upgrade, the quality and speed of image acquisition will be improved. However, the acquisition software, scan area, intensity, etc., will differ (it cannot be applied to the original LSM700 settings), and the original users must be re-qualified to use the upgraded model. The center will arrange the training course and assist in re-qualifying.
Microscope:
  • Fully motorized inverted microscope Axio Observer Z.1
  • Electric XY platform can be used for large-area map images and multi-position imaging
Objectives:
  • Fluar 5x/0.25
  • Fluar 10x/0.5 DIC
  • Plan-Apochromat 20x/0.8 DIC
  • Plan-Apochromat 40x/1.4 Oil DIC
  • Plan-Apochromat 63x/1.4 Oil DIC
  • Plan-Apochromat 100x/1.40 Oil DIC
Laser (nm):
  • Solid-state laser: 405nm, 488nm, 555nm, 639nm
Detectors:
  • Two sets of high-sensitivity PMT with fixed fluorescent filters and adjustable VSD BeamSplitter (Note 1).
  • A set of T-PMT dedicated detector for high-sensitivity transmitted light images, including automatic switching light path control, which can acquire various transmitted light images, such as bright field and differential interference contrast (DIC) images. 
Live-cell Incubation:
zeiss-poc stage-top incubatorStage-top live-cell incubation system with temperature, humidity and CO2 control module
The picture above shows [Stage-top live-cell incubation system PM S1], which is directly installed on the stage. The carbon dioxide concentration is stably controlled from 0.0% to 10.0% (±0.1%). It is suitable for glass-bottom dishes with diameters of 30-60mm and coverslip thickness of 0.17mm. If you need to use the live cell system, please contact the administrator before making your first appointment.  

Image Software:
  • Zen 2009, Confocal image acquisition and analysis ( LSM700 Operation Manual ) 
  • Offline computer workstations are available for image processing and analysis

LSM700 Scan Head
Note 1. LSM700 Scan Head:
LSM700 is equipped with two sets of PMTs and a VSD Beam Splitter to provide signal acquisition band flexibility with an adjustable accuracy of 1 nm. It is not limited by traditional filters and can collect more signals.
  • Colocalization,
  • 3D imaging,
  • Time-lapse imaging,
  • Photo Bleaching, (FRAP, FLIP, FRET, Photoactivation photoconversion),
  • Linear unmixing, etc.
*Users must be certified.
Users should pass the certification test after the instruction and operate the system more than five times (inclusive) during office hours (8:30-16:30) before they are authorized to reserve online, have access to Room 640 and can use the system according to the regulations. If the certified user does not use the device for one month, they must be certified again by the administrator.
  1. Reservation regulations:
    • Please go to the "Instrument Reservation System" to make a reservation before use. The person who makes the reservation must be the actual user.
    • After use, please register in the record book. The start time is the reservation time, and the end time is based on the end time of use. You cannot delay the start time or occupy other people's sessions.
    • Reservations cannot be canceled on the same day. Reservations made more than half an hour after the scheduled time are considered abandoned.
    • No same-day reservations are accepted. Please confirm your reservation by 16:30 of the previous workday at the latest; late changes violate the rules and will not be accepted.
    • Each person can make 4 reservations within two weeks, and each reservation can be made for 2 hours.
  2. Sample requirements:
    • Always use 0.17mm ( No.1.5) glass coverslips for slide samples or glass-bottom Petri dishes.
    • Please be sure to clean the surface of your samples before loading.
    • Please ensure the samples are sealed and a fluorescent microscope is used to confirm the samples are fluorescent before loading. If the samples cannot be serviced due to their own factors, the center will still charge according to the hours of operation.
    • If you have special needs, please inform us of the dyes used for the samples before operations so the technician can understand and advise the user's needs.
    • Please contact the center administrator for any other needs or special requirements.
  • Monday to Friday
    • All day, 00:00-24:00, appointment registration for certified users only
  • Weekends
    • Not open for use
 　　 　　 　　 　　 　　
Instrument Charges (NTD per Hour)
Confocal
Microscope		 (Z) LSM880 (Z) LSM900 (O) FV1000
(Z) LSM700		 (O) FV10i (Z) LSM7MP
  Office hours at night Living cells Fluorescence Office hours at night Living cells Fluorescence      
Restricted to Certified Users   V V   V V V V V
Fees for NYCU faculty and students 900 500 300 800 600 300 500 300 1,000
Fees for cooperative educational units 1,350 -- 450 1,200 -- 450 750 450 1,500
Fees for other unit 1,800 -- 600 1,600 -- 600 1,000 600 2,000
Remarks
  1. Abbreviations: (O) Olympus, (Z) Zeiss
  2. Number of Reservations: Each person can make up to four reservations on a single device within a two-week period. Nighttime use and LSM7MP allow up to 3 hours per reservation, while other devices allow up to 2 hours per reservation. Reservations can be made consecutively on all devices.
  3. The office hours are from 08:30 to 16:30, and the nighttime period is from 16:30 to 08:30 of the next day.
  4. If you need to take time-lapse imaging overnight, you must contact the administrator of the center in advance.
  5. LSM 7MP is open from 09:00 to 17:00. If you need to operate at night, you must inform the administrator in advance.
  6. In addition to the education and training of the imaging core facilities organized by the center, it is recommended that you prepare your own samples (experimental data can be taken away) for scheduled training and certification tests. The charges will be the same as for general use of the system.
  7. The above table shows the hourly usage charge. Charges are calculated on a half-hour basis.
  1. If you have the following qualifications, you will be charged the same fee as NYCU faculty and students:
    • Branch school
    • Joint-appointed faculty 
    • Affiliated hospitals 
    • Projects are done in cooperation with and under the control of the University.
  2. If you have the following qualifications, the fee will be the same as that of a cooperative educational unit:
    • Adjunct faculty
    • Teaching hospitals (Veterans General Hospital, Taipei City Hospital, Far Eastern Memorial Hospital, etc.)
    • University System of Taiwan (UST)
    • Companies participate in the academia-industry cooperation center of NYCU
Instruments are charged per half hour, and the billing will be counted at the end of each odd-numbered month. Please bring the bill to the Cashier's Section of the Administration Building within two weeks at the latest. The service will be stopped if the number of overdue payments reaches (and includes) two times.
  1. No USB flash drive is allowed. Please use FTP to transfer files or bring your blank CD-ROM to burn data. Due to the limited space of the computer's hard disk, the administrator will delete the data from time to time to facilitate the normal operation of the instrument. We are not responsible for data storage.
  2. Without the consent of the administrator of the Center, you are not allowed to add or remove any parts, alter the system settings, or install software on the Center's public equipment. If any experiment requires any test, the administrator's consent must be sought first and accompanied by the engineer of the original manufacturer to carry out the testing without damaging the normal operation of the equipment, and only after the passing of the test can it be operated and used by the personnel approved by the administrator of the Center under the condition of the administrator's permission.
  3. According to the administrator's judgment, the violator is warned for the first time, canceled the right to use for one month for the second time, and canceled the right to use permanently for the third time; if the violator violates the above specification, resulting in the damage of the instrument, the violator shall pay compensation to repair the instrument and stop the right to use for one month. The violator is prohibited from using the instrument permanently.
  • Certification form-confocal_2022
  • LSM700 Operation Manual 
  • User Rules for Confocal Microscopes and Advanced Optical Microscopes
    • User rules
Instrument List of YM Campus Imaging Core of YM Campus Other Confocal Microscopes
Related Image(s)：
  • Contact： [ ext. 66185]
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