Biomedical Imaging
中文- Update Date：2025-01-15
- Units：Instrumentation Resource Center
[YangMing] Small animal positron and MRI instrument: Bruker 7T PET/MR
- Model: Bruker 7T PET/MR
- Instrument Location: 2nd Floor, Old Positron Center (Cyclotron Center), Taipei Veterans General Hospital
- The independent entrance of the building is located on the hillside behind the hospital, adjacent to the Rongyang Tunnel.
- On foot, you can enter from the first floor of the Siyuan Building, take the elevator to the fifth floor of the Siyuan Building, walk towards Zhengzi Center or Spirit Building, and you will see the entrance of Positron Center.
- During the pandemic, the first floor of the Siyuan Building will not be open to traffic. Please enter from the Zhongzheng Building and connect the passage from the second floor of the Zhongzheng Building to the Siyuan Building.
- Administrator: Prof. Lin Ching-po
- Extension 67338
- Management unit: Yangming Campus Small Animal Imaging Core of Brain Research Center
- Office Location: Room 532, 5th Floor, Traditional Medicine Building A
- Email: nycu.saic@gmail.com
- On-campus extension 65293
- Line account: @268uqyax (Add as a friend)
This instrument is a 7T MRI, which can provide images with higher resolution and higher signal-to-noise ratio (SNR) than traditional 3T MRI.
Various functional imaging is possible.
In addition to generating anatomical images of brain soft tissue superior to computed tomography (CT), it can perform various functional imaging, such as brain nerve fiber orientation imaging (DTI), brain angiography, functional imaging, water molecule diffusion Imaging, and animal pathological studies. Combined with ECG and a respiration monitoring system, the artifacts caused by respiration and heartbeat shaking during imaging are minimized, and they can also be synchronized with the heartbeat cycle (Gating) to obtain dynamic cardiac images.
With PET's ability to track drug concentrations through radiation, high-resolution functional images can be obtained.
PET obtains high-resolution functional images by tracking the specific accumulation of drugs by radiation. Various functional imaging can be performed depending on the use of monitoring drugs. Such as tracking tumor location and evaluation of malignancy, tracking of the inflammatory response, and research on myocardial perfusion status and neurodegenerative diseases; dynamic imaging can be used to obtain pharmacokinetic studies of drug distribution and metabolism.
The imaging range of this PET is up to 15 cm, allowing normal-sized mouse images to be imaged in a single session.
PET and MR imaging maintain the exact position of the animal and accurately blend the two images to combine molecular-level pathology or functional structures with anatomical images. It is a new tool for non-invasive therapeutic preclinical studies, drug screening, and material research, which can assist in cross-disciplinary imaging studies of small animals.
Various functional imaging is possible.
In addition to generating anatomical images of brain soft tissue superior to computed tomography (CT), it can perform various functional imaging, such as brain nerve fiber orientation imaging (DTI), brain angiography, functional imaging, water molecule diffusion Imaging, and animal pathological studies. Combined with ECG and a respiration monitoring system, the artifacts caused by respiration and heartbeat shaking during imaging are minimized, and they can also be synchronized with the heartbeat cycle (Gating) to obtain dynamic cardiac images.
With PET's ability to track drug concentrations through radiation, high-resolution functional images can be obtained.
PET obtains high-resolution functional images by tracking the specific accumulation of drugs by radiation. Various functional imaging can be performed depending on the use of monitoring drugs. Such as tracking tumor location and evaluation of malignancy, tracking of the inflammatory response, and research on myocardial perfusion status and neurodegenerative diseases; dynamic imaging can be used to obtain pharmacokinetic studies of drug distribution and metabolism.
The imaging range of this PET is up to 15 cm, allowing normal-sized mouse images to be imaged in a single session.
PET and MR imaging maintain the exact position of the animal and accurately blend the two images to combine molecular-level pathology or functional structures with anatomical images. It is a new tool for non-invasive therapeutic preclinical studies, drug screening, and material research, which can assist in cross-disciplinary imaging studies of small animals.
Functional Brain Imaging
By using Magnetic Resonance Imaging (MRI) and Positron Emission Tomography (PET), it is possible to minimize the differences in individual posture movement during angiography and obtain functional images such as cerebral blood flow and brain inflammation. These functional images can then be superimposed with anatomical images T1 and T2 to obtain brain inflammation (FEPPA), glucose uptake (FDG), and quantitative analysis of tissue blood flow.
By using Magnetic Resonance Imaging (MRI) and Positron Emission Tomography (PET), it is possible to minimize the differences in individual posture movement during angiography and obtain functional images such as cerebral blood flow and brain inflammation. These functional images can then be superimposed with anatomical images T1 and T2 to obtain brain inflammation (FEPPA), glucose uptake (FDG), and quantitative analysis of tissue blood flow.
MRI T2 image
Cerebral Blood Flow (ASL)
PET FEPPA drug imaging
PET/MR Static and Dynamic Whole Body Imaging
The study utilized MRI and PET scans to minimize the effects of individual posture movement during imaging. A full-body image of a mouse can be obtained in a single imaging session by focusing on the local PET Z-axis and capturing images within a 15 cm range. This method facilitated precise tumor and inflammatory response analysis in animal models by combining functional and anatomical images.
- On the left is an image of a mouse suffering from rheumatoid arthritis. The inflammation at the joint location of the lesion causes a high glucose uptake, resulting in a high FDG uptake. Analyzing and quantifying the disease model can be done by aligning it with the anatomical MRI T1 image.
- On the right is an image of FEPPA, which binds to the inflammatory receptors of TSPO. This is used to evaluate inflammation models.
High-resolution Brain MRI
High-resolution T2 images are used to understand brain structure and analyze brain lesions to facilitate tracking of living animals.
Tumor Tracking
When it comes to tumor tracking, regular T1 and T2 images are used to track the actual status of tumor growth and obtain the tumor type in vivo, the effect on surrounding tissues, and the treatment effect. This helps to achieve the application of long-term tracking in vivo.
PET/MR Dynamic Cardiac Angiography
PET/MR dynamic cardiac angiography uses a system synchronized with the heartbeat and respiratory movements to acquire MRI and PET images of the heart in different cycles. This system enables us to observe the beating of the ventricle and atrium. Additionally, it can be used to determine the anatomy and function of the cardiac muscle cells based on their glucose state. To integrate the acquired images, the black and white MRI background is combined with a colored PET image, resulting in more accurate interpretation and research.
- It is open from 9 am to 5 pm, Monday to Friday (closed on holidays).
- The rest of the period will be open after further announcement, depending on the equipment use and the technicians' status.
Make an appointment
- NYCU and Taipei Veterans General Hospital users can make reservations four weeks before.
- Users from off-campus units can make reservations three weeks in advance.
- All billing and payment processes must be completed before the experiment. Otherwise, all appointments for the month will be canceled.
- Make reservations at least seven days in advance, and the session is in units of 1 hour.
- If you want to cancel the experiment, please log in to the reservation system seven days before the experiment to cancel it yourself; if you cancel 3-6 days before the experiment, half of the reservation time will be deducted; if you cancel within two days before the imaging day, the fee will be charged as usual.
- For new experiments and special requirements, please inform the operator of the experiment content one week before use.
- When the experiment exceeds the reserved time, if other units are making an appointment later, the use can be extended for up to ten minutes with the consent of the following user. If there is no subsequent appointment by other units, the user can extend the session. If the extension exceeds twenty minutes, the session will be charged. Use less than one hour is counted as one hour.
- Please arrive on time according to the reserved time slot. Latecomers are not allowed to delay operations. If you are 30 minutes late, it will be regarded as a no-show and will not be refunded for the time slot. The facility will suspend the users who are late or fail to show up repeatedly.
Specifications for Using the Instrument
- Due to the limited storage space of the computer, the image files can be kept for a maximum of 30 days. The center will not be responsible for file loss due to machine damage.
- Currently, it is open for tissue and cell samples from general animals (bred on behalf of animal centers or in a temporary room approved by the animal center.) Infectious animals and samples are temporarily not allowed to be used.
- We only provide imaging services. Analysis services, contrast agents (CT contrast agent, luminescence, etc....), anesthetics (isoflurane only provides gas anesthesia machines), and consumables (needles, black cardboard, etc....) are not provided.
- Gas anesthesia devices prohibit using gas anesthetics other than Isoflurane.
- Applying for angiography means agreeing to use Isoflurane anesthesia or administering a contrast agent during the animal angiography. The animal may die during the experiment due to physical weakness or other irresistible factors, resulting in abnormal conditions such as hypothermia and respiratory distress.
- The core facility is not responsible for keeping items deposited by users.
- Each full-time NYCU faculty member has two hours of free operation to facilitate the acquisition of preliminary research materials (including the teacher himself, who can only use the free operation quota of three teachers at most).
- Articles published in the name of NYCU and mentioned, thanks to the small animal imaging core facility of NYCU Brain Research Center, can get rewards. Each article will be deducted the usage fee equivalent to impact factor times hours (in rounds, less than one point is calculated by one point). Only one application can be made per article.
- Acknowledgments:
We thank the Molecular Imaging Facility Small Animal Instrument Name and Brain Research Center at National Yang Ming Chiao Tung University for the technical support.
| Unit | NYCU and Taipei Veterans General Hospital | other academic units | industry |
|---|---|---|---|
| usage fee | NT$2,500/hour | NT$3,000/hour | NT$5,000/hour |
- The equipment usage fee does not include the cost of luminescence, contrast agent, gas anesthesia agent, and positron contrast agent.
- The user must prepare consumables such as luminescent elements, contrast agents, and isoflurane. The small animal imaging core facility will not provide these.
- Positron contrast agent is provided and charged by the Positron Center of Taipei Veterans General Hospital. The small animal imaging core facility will assist with the application. Positron Contrast Agent Application Process
- Application process
- Positron Radiopharmaceutical Application Form
- Application And Payment Instructions For Positron Radiopharmaceuticals
| Routine medication (times) | Taipei Veterans General Hospital/Yang Ming | other academic units | industry |
|---|---|---|---|
| [18F] FDG | 3,000 | 3,900 | 6,000 |
| [18F] FBPA [18F] NaF [11C] Sodium acetate [18F] NH3 |
7,500 | 9,750 | 15,000 |
| Special medication (times) | Taipei Veterans General Hospital/Yang Ming | other academic units | industry |
|---|---|---|---|
| [11C] PIB [18F] FEPPA [18F] FAHA 4-[18F] ADAM [18F] FHBG [18F] THK5351 |
7,500 | 9,750 | 15,000 |
| Newly developed drugs | 15,000 | 19,500 | 30,000 |
- The Nuclear Medicine Department of Taipei Veterans General Hospital will collect the charges for positron radiopharmaceuticals. It cannot be combined with the usage fees.
- If there is any error or change, the announcement of the Nuclear Medicine Department of Taipei Veterans General Hospital shall be the mainstay.
- Website of The Nuclear Medicine Department of Taipei Veterans General Hospital
Payment method (please follow the purchase method and order)
By Cash
By Cash
- Fill out the User Consent Certificate in duplicate and ask the project host to sign or seal it.
- Bring the user consent certificate (in duplicate) to the cashier group to pay (stamp, take receipt).
- Bring the User Consent Certificate (in duplicate), payment receipt, and IACUC certificate to the Small Animal Imaging Core Facility to log in for hours.
By Check
- Fill out the User Consent Certificate in duplicate and ask the project host to sign or seal it.
- Bring the cheque and the user consent certificate (in duplicate) to the cashier team to pay (stamp, take the receipt).
- Bring the User Consent Certificate (in duplicate), payment receipt, and IACUC certificate to the Small Animal Imaging Core Facility to log in for hours.
On-Campus Loan Methods
- Fill in the user certificate in duplicate, ask the project host to sign or seal it, and fill in the school loan slip in the accounting requisition system.
(Pre-loan application and write-off → payee details → beneficiary code key in "000" → NYCU internal transfer). - Prepare documents of on-campus loan slip and user consent certificate to the Small animal imaging core facility Office - Stamped (the stamped certificate is the loan voucher).
- After completing item (2), hold the on-campus loan receipt and loan voucher (with small animal imaging core facility certificate after the office seal) to the Accounting Office to apply for the loan.
- After completing item (3), wait approximately three to four weeks for the cashier team to notify you to collect your receipt.
Please prepare the following documents: the original copy of the user certificate (in duplicate) and the cashier's seal. - Present receipt, User Consent Certificate (in duplicate) stamped by the Cashier team, and IACUC attestation to the Small animal imaging core facility to log in for hours.
- Reference document for sending a letter of inter-school remittance
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