- <![CDATA[[YangMing] General Purpose Benchtop Centrifuge]]><![CDATA[<div class="ed_model05 clearfix">
<div class="ed_pic_right"><img alt="Sorvall ST4 Plus" src="/userfiles/ordch/images/20240123150813391.jpg" /></div>
<ul>
<li class="ed_txt"><strong>General Purpose Benchtop Centrifuge</strong></li>
<li class="ed_txt"><strong>Brand: </strong>Thermo Scientific</li>
<li class="ed_txt"><strong>Model: </strong>Sorvall ST4 Plus</li>
<li class="ed_txt"><strong>Instrument Location</strong>: Yang-Ming Campus
<ul>
<li class="ed_txt"><strong>Room615, Library, Information & Research Building <span style="color:#8e44ad;">(Machine A)</span><span style="color:#ff0000;">*</span></strong></li>
</ul>
</li>
<li class="ed_txt"><strong>Term of use： </strong>
<ul>
<li class="ed_txt">On-Demand, No reservation required.</li>
<li class="ed_txt">Free of charge for NYCU users. Off-campus users, please contact the center before using it.</li>
</ul>
</li>
<li class="ed_txt"><strong>Contact us:</strong>
<ul>
<li class="ed_txt"><strong>Extension：</strong>62382</li>
<li class="ed_txt"><strong>E-mail：</strong><a href="mailto:ycirc@nycu.edu.tw" title="ycirc@nycu.edu.tw">ycirc@nycu.edu.tw</a></li>
</ul>
</li>
</ul>
</div>]]>Instrumentation Resource Center 2025-01-15Introduction<span style="font-size:105%;"><strong>Specification</strong></span>
<ul>
<li>Control System: automatic microcomputer control, large LCD display, can set and display speed, time, temperature, acceleration/deceleration, rpm, rcf value conversion, and bucket certification code independently.</li>
<li>Auto-Lock rotor locking system</li>
<li>Program group: 6 groups</li>
<li>Temperature setting: -10℃ ~ +40℃, with pre-cooling function.</li>
<li>Freezing system: Fast freezing, reach 4℃ in 15 minutes.</li>
<li>Quick centrifugal function</li>
<li>Acceleration/deceleration setting: 9/10 segments</li>
<li>A wide range of applications can be expanded to 12 rotors for replacement.</li>
</ul>
<div class="table 03" style="ed_table caption-top">
<table align="center" border="1" class="ed_table caption-top">
<caption>Currently equipped with these rotors</caption>
<tbody>
<tr>
<td style="text-align: center;">Rotor</td>
<td style="text-align: center;"><a href="https://www.thermofisher.com/order/catalog/product/75003180" rel="noreferrer noopener" target="_blank" title="TX-750 Swinging Bucket Rotor with Round Buckets(Open New Windows)"><strong>TX-750 Swinging Bucket Rotor with Round Buckets<br />
<img alt="TX-750 Swinging Bucket Rotor " src="/userfiles/orden/images/20241203112611771.jpg" /></strong></a></td>
<td style="text-align: center;"><a href="https://www.thermofisher.com/order/catalog/product/75003624?SID=srch-srp-75003624" rel="noreferrer noopener" target="_blank" title="M-20 Microplate Swinging Bucket Rotor(Open New Windows)"><strong>M-20 Microplate Swinging Rotor and Buckets<br />
<img alt="M-20 Microplate Swinging Bucket Rotor" src="https://www.nycu.edu.tw/userfiles/ordch/images/20241203165353801.jpg" /></strong></a></td>
</tr>
<tr>
<td style="text-align: center;">Maximum Capacity</td>
<td data-th="TX-750" style="text-align: center;">4×750ml</td>
<td data-th="M-20" style="text-align: center;">2 x 3 Standard or 2 x 1 Deepwell Plates</td>
</tr>
<tr>
<td style="text-align: center;">Maximum Speed</td>
<td data-th="TX-750" style="text-align: center;">4,700 rpm</td>
<td data-th="M-20" style="text-align: center;">4,000 rpm</td>
</tr>
<tr>
<td style="text-align: center;">Maximum Centrifugal Force</td>
<td data-th="TX-750" style="text-align: center;">4,816 xg</td>
<td data-th="M-20" style="text-align: center;">2,272 xg</td>
</tr>
<tr>
<td style="text-align: center;">Adapter</td>
<td data-th="TX-750">
<ul>
<li style="text-align: center;">Maximum 28 pieces of 50ml pointed-bottom centrifuge tube at a time</li>
<li style="text-align: center;">Maximum 56 pieces of 15ml pointed-bottom centrifuge tube at a time</li>
</ul>
<img alt="TX-750 tube adaptors" src="/userfiles/ordch/images/20241203151106702.png" /></td>
<td data-th="M-20" style="text-align: center;">--</td>
</tr>
</tbody>
</table>
</div>Rules of Use<div class="ed_model01 clearfix">
<ol>
<li class="ed_txt">Please read the instructions carefully before use and contact the center at extension 62382 if you have any questions.</li>
<li class="ed_txt">Please record the usage information in the registration book.</li>
<li class="ed_txt">The Centrifuge is currently equipped with
<ol start="1" style="list-style-type: upper-alpha;">
<li class="ed_txt">the <a href="https://www.thermofisher.com/order/catalog/product/75003180" rel="noreferrer noopener" target="_blank" title="TX-750 Swinging Bucket Rotor(開啟新視窗)">TX-750 Swinging Bucket Rotor</a>, which can be used in centrifuge cups with the adapter for 15ml or 50ml tubes: yellow adapters for 15ml tubes, green adapters for 50ml tubes, and make sure to maintain symmetry. <img alt="tube adaptor placement" src="/userfiles/ordch/images/20241202144113139.jpg" /></li>
<li class="ed_txt">the <a href="https://www.thermofisher.com/order/catalog/product/75003624?SID=srch-srp-75003624" rel="noreferrer noopener" target="_blank" title="M-20 Microplate Swinging Bucket Rotor(Open New Windows)">M-20 Microplate Swinging Bucket Rotor</a>, which can hold up to six standard microplates. <strong>To ensure safe operation of the centrifuge, the rotor must be evenly loaded at all times!<img alt="M20 microplate swinging bucket rotor loading" src="https://www.nycu.edu.tw/userfiles/ordch/images/20241202153042627.png" /> </strong></li>
</ol>
</li>
</ol>
</div>Manuals<div class="ed_model17 clearfix ed_list">
<ul>
<li class="d-flex">ST4R Quick start (Chinese version)
<ul class="d-flex">
<li> </li>
<li><a href="/userfiles/ordch/files/20240123154712895.pdf" target="_blank" title="Quick start(Open New Windows)"><img alt="Quick start" src="/userfiles/file/pdf.png" /> </a></li>
<li> </li>
</ul>
</li>
<li class="d-flex">User Manual (English version)
<ul class="d-flex">
<li><a href="/userfiles/orden/files/20240715110202361.pdf" target="_blank" title="Manual(Open New Windows)"><img alt="Manual" src="/userfiles/file/pdf.png" /> </a></li>
<li> </li>
</ul>
</li>
</ul>
</div>Related Links<div class="ed_model01 clearfix">
<div class="ed_txt"><a href="https://www.thermofisher.com/order/catalog/product/75016085?SID=srch-srp-75016085" target="_blank" title="Sorvall ST4 Plus Centrifuge Series(開啟新視窗)"><img alt="Sorvall ST4 Plus Centrifuge Series" src="/userfiles/ordch/images/20231017084212978.png" /></a><br />
<br />
Currently Equipped with two rotors:</div>
<ol>
<li class="ed_txt"><a href="https://www.thermofisher.com/order/catalog/product/75003180" rel="noreferrer noopener" target="_blank" title="TX-750 Swinging Bucket Rotor(Open New Windows)">TX-750 Swinging Bucket Rotor</a></li>
<li class="ed_txt"><a href="https://www.thermofisher.com/order/catalog/product/75003624?SID=srch-srp-75003624" rel="noreferrer noopener" target="_blank" title="M-20 Microplate Rotor and Buckets(Open New Windows)">M-20 Microplate Rotor and Buckets</a> </li>
</ol>
</div>https://ord.nycu.edu.tw/ord/en/app/machine/image?module=machine&detailNo=1199282243734867968&init=YSorvall ST4 Plushttps://ord.nycu.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2023&serno=da69be39-dc4e-48d6-9168-7c96b2dbdb6eInstrument List of YM Campushttps://ord.nycu.edu.tw/ord/en/app/machine/list?module=machine&id=2058Other Centrifuges
- <![CDATA[[YangMing] High-Speed Centrifuge]]><![CDATA[<!-- 直式圖片(調整圖片高度，400px數字可自行依照左側文字適合高度調整) -->
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<div class="ed_model05 clearfix">
<div class="ed_pic_right"><img alt="high-speed centrifuge" src="/userfiles/ordch/images/20231018130115380.png" /></div>
<ul>
<li class="ed_txt"><strong>High-Speed Centrifuge</strong></li>
<li class="ed_txt"><strong>Brand/Model: </strong>Beckman Coulter
<ul>
<li class="ed_txt"><span style="color:#3498db;"><strong>Avanti_J-25</strong></span>: F, G and H machine</li>
<li class="ed_txt"><span style="color:#3498db;"><strong>Avanti_J-26 XP:</strong></span><strong> </strong>I, J and K machine</li>
</ul>
</li>
<li class="ed_txt"><strong>Instrument Location</strong>: Yang-Ming Campus
<ul>
<li class="ed_txt"><strong>Room 465-2, Biomedical Engineering Building <span style="color:#8e44ad;">(F), (I)</span></strong></li>
<li class="ed_txt"><strong>Room 710-1, Biomedical Building<span style="color:#8e44ad;"> (G), (J) </span></strong></li>
<li class="ed_txt"><strong>Room </strong><strong>615, Library, Information & Research Building <span style="color:#8e44ad;">(K) </span></strong></li>
<li class="ed_txt"><strong>Room B02, Shou-Ren Building<span style="color:#8e44ad;"> (H)</span></strong></li>
</ul>
</li>
<li class="ed_txt"><strong>Term of Use: </strong>
<ul>
<li class="ed_txt"><span style="color:#e74c3c;">Users must pass the examination and certification to operate. Available to use on demand.</span> </li>
<li class="ed_txt">Free of charge for NYCU users. Off-campus users, please contact the center before using it.</li>
</ul>
</li>
<li class="ed_txt"><strong>Contact Center Staff:</strong>
<ul>
<li class="ed_txt"><strong>Extension: </strong>62382</li>
<li class="ed_txt"><strong>Email: </strong><a href="mailto:ycirc@nycu.edu.tw" title="ycirc@nycu.edu.tw">ycirc@nycu.edu.tw</a></li>
</ul>
</li>
</ul>
</div>]]>Instrumentation Resource Center 2025-01-15#62382Introduction<div class="ed_model01 clearfix">
<div class="ed_txt"><strong>High-Speed Centrifuge</strong><br />
<br />
We have two series of high-speed centrifuges available in the instrument center: the Avanti J-25 and Avanti J-26 models. The maximum speeds for these models are 25,000rpm (75,600g) and 26,000rpm (81,770g) respectively. Although there are slight differences in centrifugal force and operation mode between the two models, their applications are similar.<br />
<br />
High-speed centrifuges are typically used for the preliminary purification of samples. These samples are often large volumes and contain a high level of impurities. When using this instrument, users usually refer to methods found in literature or experimental procedures. Select the appropriate rotor and centrifuge bottle based on the required centrifugal force and sample volume to achieve better experimental results.<br />
<br />
Furthermore, since different samples are dissolved in different buffers with varying pH and other physical chemistry properties, it is essential to carefully select centrifuge bottles made of various materials to prevent breakage during centrifugation. This is crucial for ensuring the safety of personnel and instruments.</div>
</div>Rules of Use<div class="ed_model01 clearfix">
<div class="ed_txt"><strong><span style="color:#ff0000;">This instrument can only be used after passing the examination and certification.</span></strong></div>
<ul>
<li class="ed_txt">After watching the video "<a href="https://www.youtube.com/watch?v=ZkK0l9-YPMc" rel="noreferrer noopener" target="_blank" title="Basic Principles of Centrifuge(Open New Windows)">Basic Principles of Centrifuge</a>," you must call the center at least 3 days in advance (on-campus extension 62382) to make an appointment with the center staff to take a written test (you can bring your own notes for reference). Only those who pass the test with a score of 80 or above can participate in the center staff's hands-on teaching. Only those who have completed the above procedures will be qualified to operate the centrifuge.</li>
<li class="ed_txt"><strong>Written test location:</strong> the Instrumentation Resource Center office on the 4th floor, Biomedical Engineering Building, or the 7th floor, Biomedical Building. Extension. 62382.</li>
</ul>
<div class="ed_txt"><strong>Rules of Use</strong></div>
<ol>
<li class="ed_txt">High-speed centrifuges should be reserved for priority use. If no reservation is made, they will be available on a first-come, first-served basis. The use of the centrifuges must be registered and should not be interrupted for more than 20 minutes. This is to ensure that other users have a fair chance to use the equipment. Otherwise, it will be considered as giving up the right to use the centrifuges. </li>
<li class="ed_txt">Reservations are limited to 2 sessions (including the in-use session), with a maximum duration of 1 hour per session and no interruptions of more than 20 minutes. </li>
<li class="ed_txt">Please remember to record your name, time of use, conditions, and ROTOR model number in the registration book.</li>
<li class="ed_txt">Store the centrifugal rotor in a refrigerator at 4℃. Please return and invert it to its original position according to the model and serial numbers.</li>
<li class="ed_txt">If the instrument or accessories are dirty after use, please clean them to maintain the equipment properly. </li>
<li class="ed_txt">If improper use causes damage to the instrument, rotor, or other accessories, the user will be responsible for paying the compensation price.</li>
</ol>
</div>Video & References<!-- 參考資料en -->
<div class="ed_model17 clearfix ed_list">
<ul>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Rotors, Tubes, and Accessories</span></span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231018130544553.pdf" rel="noreferrer noopener" target="_blank" title="Rotors and Tubes(Open New Windows)"><img alt="Rotors and Tubes" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex">High-Speed Centrifuge J-26 XP Manual
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231018130658115.pdf" rel="noreferrer noopener" target="_blank" title="J-26 XP Manual(Open New Windows)"><img alt="J-26 XP Manual" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Build your Centrifuge Configuration: Tube/Bottle/Rotor Calculator</span> </span>
<ul class="d-flex">
<li><a href="https://www.beckman.tw/centrifuges/rotors/calculator" rel="noreferrer noopener" target="_blank" title="Build your Centrifuge Configuration(Open New Windows)"><img alt="Build your Centrifuge Configuration Tube/Bottle/Rotor Calculator" src="/userfiles/file/link.png" /> </a></li>
</ul>
</li>
</ul>
</div>
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<div class="embed-container"><strong>Basic Principles of Centrifuges（ Play 30:05 ）</strong><br />
<iframe allowfullscreen="" frameborder="0" sandbox="allow-scripts allow-same-origin" src="https://www.youtube.com/embed//ZkK0l9-YPMc"></iframe></div>Related Links<div class="ed_model01 clearfix">
<div class="ed_txt"><a href="https://www.beckman.com/centrifuges/high-speed" rel="noreferrer noopener" target="_blank" title="BECKMAN COULTER(Open New Windows)"><img alt="BECKMAN COULTER" src="/userfiles/ordch/images/20231017135324365.jpg" /></a></div>
</div>https://ord.nycu.edu.tw/ord/en/app/machine/image?module=machine&detailNo=1167351046968709120&init=YHigh-Speed Centrifugehttps://ord.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2023&serno=da69be39-dc4e-48d6-9168-7c96b2dbdb6eInstrument List of YM Campushttps://www.nycu.edu.tw/ord/en/app/machine/list?module=machine&id=2058Other Centrifuges
- <![CDATA[[YangMing] Tabletop Ultracentrifuge]]><![CDATA[<style type="text/css">.ed_model11 .job-title {
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<div class="ed_model05 clearfix">
<div class="ed_pic_right"><a href="/userfiles/ordch/images/20240219172850917.jpg" target="_blank" title="Tabletop Ultracentrifuge(Open New Windows)"><img alt="Tabletop Ultracentrifuge" src="/userfiles/orden/images/20240219173458879.jpg" /></a></div>
<ul>
<li class="ed_txt"><strong>Tabletop Ultracentrifuge</strong></li>
<li class="ed_txt"><strong>Brand/Model: </strong>Beckman Coulter
<ul>
<li class="ed_txt">TL-100: M machine</li>
<li class="ed_txt">TLX: L machine</li>
<li class="ed_txt">MAX-XP: O machine</li>
</ul>
</li>
<li class="ed_txt"><strong>Instrument Location</strong>: Yang-Ming Campus
<ul>
<li class="ed_txt"><strong>Room 465-2, Biomedical Engineering Building <span style="color:#8e44ad;">(M machine)</span> </strong></li>
<li class="ed_txt"><strong>Room 710-1, Biomedical Building <span style="color:#8e44ad;">(L machine)</span><span style="color:#ff0000;">*</span> </strong></li>
<li class="ed_txt"><strong>Room B02, Shou-Ren Building<span style="color:#8e44ad;"> (O machine)</span><span style="color:#ff0000;">*</span></strong></li>
</ul>
</li>
<li class="ed_txt"><strong>Term of Use: The user must pass the examination and certification to use and must make an appointment in advance.</strong>
<ul>
<li class="ed_txt"><strong><span style="color:#ff0000;">Those marked with * must make an online reservation. The others are paper reservations.</span></strong></li>
<li class="ed_txt">Free of charge for NYCU users. Off-campus users, please contact the center before using it.</li>
</ul>
</li>
<li class="ed_txt"><strong>Reservation >>> <a href="https://ircbooking.nycu.edu.tw/IRC/irc_booking/index_en.aspx" rel="noreferrer noopener" target="_blank" title="Instrument Reservation System(Open New Windows)">Instrument Reservation System</a></strong></li>
<li class="ed_txt"><strong>Contact center staff：</strong>
<ul>
<li class="ed_txt"><strong>Extension：</strong>62382</li>
<li class="ed_txt"><strong>Email：</strong><a href="mailto:ycirc@nycu.edu.tw" title="ycirc@nycu.edu.tw">ycirc@nycu.edu.tw</a></li>
</ul>
</li>
</ul>
</div>]]>Instrumentation Resource Center 2025-01-15#62382Introduction<div class="ed_model01 clearfix">
<div class="ed_txt"><strong>Ultracentrifuge</strong><br />
In most cases, samples that are to be sent to the ultracentrifuge have already undergone some initial purification steps. After that, they need to undergo additional steps to obtain purer samples with ultracentrifuge.<br />
<br />
Ultracentrifuges are equipped with four types of rotors to meet different sample requirements:</div>
<ul>
<li class="ed_txt"><strong>Fixed Angle Rotor</strong>: Mainly used for rapid separation of plasma DNA, subcellular particles, and fractions, as well as lipoproteins, viruses, and microsomes.</li>
<li class="ed_txt"><strong>Vertical-Tube Rotor:</strong> This rotor is mainly used for rapid separation of the density gradient, a technique where particles are separated based on their density; isopycnic centrifugation, a method that separates particles of equal density, and rate-zonal centrifugation, a process that separates particles based on their sedimentation rate.</li>
<li class="ed_txt"><strong>Swinging-Bucket Rotor</strong>: Mainly used in rate-zonal or isopycnic centrifugation of nucleic acid, protein, and virus.</li>
<li class="ed_txt"><strong>Near-Vertical Rotor</strong>: Mainly used for rapid separation of lipoproteins from mitochondrial DNA or plasma DNA in CsCl gradients.</li>
</ul>
<div class="ed_txt">Users can choose the appropriate rotor based on the experimental design of the sample separation to achieve the best experimental results.</div>
</div>Rules of Use<div class="ed_model01 clearfix">
<div class="ed_txt"><strong><span style="color:#ff0000;">This instrument can only be used after passing the examination and certification.</span></strong></div>
<ul>
<li class="ed_txt">After watching the video "<a href="https://www.youtube.com/watch?v=ZkK0l9-YPMc" rel="noreferrer noopener" target="_blank" title="Basic Principles of Centrifuge(Open New Windows)">Basic Principles of Centrifuge</a>", you must call the center at least 3 days in advance (on-campus extension 62382) to make an appointment with the center staff to take a written test (you can bring your own notes for reference). Only those who pass the test with a score of 80 or above can participate in the center staff's hands-on teaching. Only those who have completed the above procedures will be qualified to operate the centrifuge.</li>
<li class="ed_txt"><strong>Written test location:</strong> the Instrumentation Resource Center office on the 4th floor, Biomedical Engineering Building, or the 7th floor, Biomedical Building. Extension. 62382.</li>
</ul>
<div class="ed_txt"><strong>Rules of Use</strong></div>
<ul>
<li class="ed_txt">The ultracentrifuge must be booked in the registration book or the <a href="https://ircbooking.nycu.edu.tw/IRC/irc_booking/index_en.aspx" rel="noreferrer noopener" target="_blank" title="online booking system(Open New Windows)">online booking system</a> before use. Each person can use the centrifuge for no more than 48 hours and 2 units at a time.</li>
<li class="ed_txt">The type and serial number of the rotor (e.g. TLA100.2, #12345) must be written on the registration book.</li>
<li class="ed_txt">The rotors should be stored in a 4℃ freezer, cleaned after use, inverted, and returned to its original position according to the model and serial numbers.</li>
</ul>
</div>Video & References<!-- 參考資料en -->
<div class="ed_model17 clearfix ed_list">
<ul>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Tabletop Ultracentrifuge Rotors and Tubes</span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231018125600531.pdf" rel="noreferrer noopener" target="_blank" title="Tabletop Rotors and Tubes(pdf)(Open New Windows)"><img alt="Tabletop Rotors and Tubes" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">MLA-130 Fixed-Angle Rotor</span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231018125655144.pdf" rel="noreferrer noopener" target="_blank" title="MLA-130 Fixed-Angle Rotor(pdf)(Open New Windows)"><img alt="MLA-130 Fixed-Angle Rotor" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Build your Centrifuge Configuration: Tube/Bottle/Rotor Calculator</span> </span>
<ul class="d-flex">
<li><a href="https://www.beckman.tw/centrifuges/rotors/calculator" rel="noreferrer noopener" target="_blank" title="Build your Centrifuge Configuration(Open New Windows)"><img alt="Build your Centrifuge Configuration Tube/Bottle/Rotor Calculator" src="/userfiles/file/link.png" /> </a></li>
</ul>
</li>
</ul>
</div>
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<div class="embed-container"><strong>Basic Principles of Centrifuges（ 線上播放 30:05 ）</strong><br />
<iframe allowfullscreen="" frameborder="0" sandbox="allow-scripts allow-same-origin" src="https://www.youtube.com/embed//ZkK0l9-YPMc"></iframe></div>Related Links<div class="ed_model01 clearfix">
<div class="ed_txt"><a href="https://www.beckman.com/centrifuges/ultracentrifuges" rel="noreferrer noopener" target="_blank" title="BECKMAN COULTER(open new windows)"><img alt="BECKMAN COULTER" src="/userfiles/ordch/images/20231017135324365.jpg" /></a></div>
</div>https://ord.nycu.edu.tw/ord/en/app/machine/image?module=machine&detailNo=1209070118529667072&init=YTabletop Ultracentrifugehttps://ord.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2023&serno=da69be39-dc4e-48d6-9168-7c96b2dbdb6eInstrument List of YM Campushttps://www.nycu.edu.tw/ord/en/app/machine/list?module=machine&id=2058Other Centrifuges
- <![CDATA[[YangMing] Ultracentrifuge]]><![CDATA[<div class="ed_model05 clearfix">
<div class="ed_pic_right"><img alt="Ultracentrifuge" src="/userfiles/orden/images/20240219170843478.jpg" /></div>
<ul>
<li class="ed_txt"><strong>Ultracentrifuge</strong></li>
<li class="ed_txt"><strong>Brand/Model: Beckman Coulter</strong>
<ul>
<li class="ed_txt"><strong><span style="color:#3498db;">L-90K</span></strong>: K machine, N machine</li>
<li class="ed_txt"><strong><span style="color:#3498db;">XL-90</span></strong>:<strong><span style="color:#3498db;"> </span></strong>F machine, G machine, H machine</li>
<li class="ed_txt"><strong><span style="color:#3498db;">XE-90</span></strong>: P machine</li>
</ul>
</li>
<li class="ed_txt"><strong>Instrument Location</strong>: Yang-Ming Campus
<ul>
<li class="ed_txt"><strong>Room 465-2, Biomedical Engineering Building <span style="color:#8e44ad;">(H)</span><span style="color:#ff0000;">*</span><span style="color:#8e44ad;">, (K)</span></strong><span style="color:#ff0000;">*</span></li>
<li class="ed_txt"><strong>Room 710-1, Biomedical Building <span style="color:#8e44ad;">(G)</span><span style="color:#ff0000;">* </span></strong></li>
<li class="ed_txt"><strong>Room 615, Library, Information & Research Building <span style="color:#8e44ad;">(N)</span> </strong></li>
<li class="ed_txt"><strong>Room B02, Shou-Ren Building <span style="color:#8e44ad;">(P)</span><span style="color:#ff0000;">*</span></strong></li>
</ul>
</li>
<li class="ed_txt"><strong>Term of Use: The user must pass the examination and certification to use and must make an appointment in advance.</strong><strong>Reservation >>> <a href="https://ircbooking.nycu.edu.tw/IRC/irc_booking/index_en.aspx" rel="noreferrer noopener" target="_blank" title="Instrument Reservation System(Open New Windows)">Instrument Reservation System</a></strong>
<ul>
<li class="ed_txt"><span style="color:#ff0000;"><strong>Those marked with * must make an online reservation. The others are paper reservations.</strong></span></li>
<li class="ed_txt">Free of charge for NYCU users. Off-campus users, please contact the center before using it.</li>
</ul>
</li>
<li class="ed_txt"><strong>Contact Center Staff:</strong>
<ul>
<li class="ed_txt"><strong>Extension: </strong>62382</li>
<li class="ed_txt"><strong>Email:</strong><a href="mailto: ycirc@nycu.edu.tw" title=" ycirc@nycu.edu.tw"> ycirc@nycu.edu.tw</a></li>
</ul>
</li>
</ul>
</div>]]>Instrumentation Resource Center 2025-01-15#62382Introduction<div class="ed_model01 clearfix">
<div class="ed_txt"><strong>Ultracentrifuge</strong><br />
In most cases, samples that are to be sent to the ultracentrifuge have already undergone some initial purification steps. After that, they need to undergo additional steps to obtain purer samples with ultracentrifuge.<br />
<br />
Ultracentrifuges are equipped with four types of rotors to meet different sample requirements:</div>
<ul>
<li class="ed_txt"><strong>Fixed Angle Rotor</strong>: Mainly used for rapid separation of plasma DNA, subcellular particles, and fractions, as well as lipoproteins, viruses, and microsomes.</li>
<li class="ed_txt"><strong>Vertical-Tube Rotor:</strong> This rotor is mainly used for rapid separation of the density gradient, a technique where particles are separated based on their density; isopycnic centrifugation, a method that separates particles of equal density, and rate-zonal centrifugation, a process that separates particles based on their sedimentation rate.</li>
<li class="ed_txt"><strong>Swinging-Bucket Rotor</strong>: Mainly used in rate-zonal or isopycnic centrifugation of nucleic acid, protein, and virus.</li>
<li class="ed_txt"><strong>Near-Vertical Rotor</strong>: Mainly used for rapid separation of lipoproteins from mitochondrial DNA or plasma DNA in CsCl gradients.</li>
</ul>
<div class="ed_txt">Users can choose the appropriate rotor based on the experimental design of the sample separation to achieve the best experimental results.</div>
</div>Rules of Use<div class="ed_model01 clearfix">
<div class="ed_txt"><strong><span style="color:#ff0000;">This instrument can only be used after passing the examination and certification.</span></strong></div>
<ul>
<li class="ed_txt">After watching the video "<a href="https://www.youtube.com/watch?v=ZkK0l9-YPMc" rel="noreferrer noopener" target="_blank" title="Basic Principles of Centrifuge(Open New Windows)">Basic Principles of Centrifuge</a>," you must call the center at least 3 days in advance (on-campus extension 62382) to make an appointment with the center staff to take a written test (you can bring your notes for reference). Only those who pass the test with a score of 80 or above can participate in the center staff's hands-on teaching. Only those who have completed the above procedures will be qualified to operate the centrifuge.</li>
<li class="ed_txt"><strong>Written test location:</strong> the Instrumentation Resource Center office on the 4th floor, Biomedical Engineering Building, or the 7th floor, Biomedical Building. Extension. 62382.</li>
</ul>
<div class="ed_txt"><strong>Rules of Use</strong></div>
<ul>
<li class="ed_txt">To ensure fair usage, the ultracentrifuge must be booked in the registration book or the <a href="https://ircbooking.nycu.edu.tw/IRC/irc_booking/index_en.aspx" rel="noreferrer noopener" target="_blank" title="online booking system(Open New Windows)">online booking system</a> before use. Each person can use the centrifuge for no more than 48 hours and 2 units at a time.</li>
<li class="ed_txt">The type and serial number of the rotor (e.g. Ti70.1, #1905) must be written on the registration book.</li>
<li class="ed_txt">The rotors should be stored in a 4℃ freezer, cleaned after use, inverted, and returned to their original position according to the model and serial numbers.</li>
</ul>
</div>Video & References<!-- 參考資料en -->
<div class="ed_model17 clearfix ed_list">
<ul>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Ultracentrifuge Rotors and Tubes</span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231018124557693.pdf" rel="noreferrer noopener" target="_blank" title="Ultracentrifuge Rotors and Tubes(pdf)(Open New Windows)"><img alt="Ultracentrifuge Rotors and Tubes" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Build your Centrifuge Configuration: Tube/Bottle/Rotor Calculator</span> </span>
<ul class="d-flex">
<li><a href="https://www.beckman.tw/centrifuges/rotors/calculator" rel="noreferrer noopener" target="_blank" title="Build your Centrifuge Configuration(Open New Windows)"><img alt="Build your Centrifuge Configuration Tube/Bottle/Rotor Calculator" src="/userfiles/file/link.png" /> </a></li>
</ul>
</li>
</ul>
</div>
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<div class="embed-container"><strong>Basic Principles of Centrifuges（ 線上播放 30:05 ）</strong><br />
<iframe allowfullscreen="" frameborder="0" sandbox="allow-scripts allow-same-origin" src="https://www.youtube.com/embed//ZkK0l9-YPMc"></iframe></div>Related Links<div class="ed_model01 clearfix">
<div class="ed_txt"><a href="https://www.beckman.com/centrifuges/ultracentrifuges" rel="noreferrer noopener" target="_blank" title="BECKMAN COULTER(開啟新視窗)"><img alt="BECKMAN COULTER" src="/userfiles/ordch/images/20231017135324365.jpg" /></a></div>
</div>https://ord.nycu.edu.tw/ord/en/app/machine/image?module=machine&detailNo=1179690955326885888&init=YUltracentrifugehttps://ord.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2023&serno=da69be39-dc4e-48d6-9168-7c96b2dbdb6eInstrument List of YM Campushttps://www.nycu.edu.tw/ord/en/app/machine/list?module=machine&id=2058Other Centrifuges