<![CDATA[[YangMing] Super Resolution Microscope with Lattice SIM² - Zeiss Elyra 7]]><![CDATA[<div class="ed_model05 clearfix">
<div class="ed_pic_right"><img alt="Super-resolution fluorescence microscope with living cell culture system Zeiss Elyra 7 with Lattice SIM2" src="/userfiles/ordch/images/20231013110037378.jpg" /></div>
<div class="ed_model01 clearfix">
<ul style="list-style-type: disc;">
<li class="ed_txt"><strong>Super-Resolution Microscope with Lattice SIM² and Incubation System</strong></li>
<li class="ed_txt"><strong>Instrument Location: </strong>Yang-Ming Campus
<ul>
<li class="ed_txt"><strong>Room 617, 6th Floor, Library, Information & Research Building</strong></li>
</ul>
</li>
<li class="ed_txt"><strong>Administrator: YM Campus Imaging Core Facility</strong>
<ul>
<li class="ed_txt">Ms. Pei-Jun Chen <a href="mailto:pjchen3@nycu.edu.tw" title="pjchen3@nycu.edu.tw">pjchen3@nycu.edu.tw</a></li>
<li class="ed_txt">Ms. Yung-yu Lu <a href="mailto:yungyu@nycu.edu.tw" title="yungyu@nycu.edu.tw">yungyu@nycu.edu.tw</a></li>
</ul>
</li>
<li class="ed_txt"><strong>Term of use: Certification</strong>
<ul>
<li class="ed_txt">You must attend the Principles and Instruments training course, have confocal hands-on experience, and undergo hands-on instruction by the Specialist before applying for the certification test.</li>
<li class="ed_txt">Only those who have passed the certification examination and operated the device more than 5 times (inclusive) during office hours will be authorized to make a reservation.</li>
</ul>
</li>
<li class="ed_txt"><strong><span style="color:#ff0000;">NT$1,300 per hour</span> for NYCU users. Please refer to the charging standard for off-campus use.</strong></li>
<li class="ed_txt"><strong>Reservation >>> <a href="https://ircbooking.nycu.edu.tw/IRC/irc_booking/index_en.aspx" rel="noreferrer noopener" target="_blank" title="Instrument Reservation System(Open New Windows)">Instrument Reservation System</a></strong> </li>
<li class="ed_txt"><strong>Please fill out the <a href="http://forms.gle/k1hr3wtmjj8G85am8" title="sample information Questionnaire">sample information Questionnaire</a> for first-time users and wait for the specialist's reply to confirm the time. </strong></li>
<li class="ed_txt"><strong>Sample testing: </strong>Each laboratory is limited to one reservation for the same type of samples (up to two hours of testing/teaching credit), and repeated reservations will be charged according to the time of use!</li>
</ul>
</div>
</div>]]>Instrumentation Resource Center 2026-04-29ext. 66185Instrument Specifications<div class="ed_model01 clearfix">
<div class="ed_txt"><strong>Microscope:</strong></div>
<ul style="list-style-type: disc;">
<li class="ed_txt">Motorized Inverted Microscope Axio Observer 7 SP</li>
<li class="ed_txt">With Piezo xyz-stage</li>
</ul>
<div class="ed_txt"><strong>Objectives:</strong></div>
<ul>
<li class="ed_txt">10X (Apotome)</li>
<li class="ed_txt">20X (Apotome)</li>
<li class="ed_txt">40X oil (Apotome)</li>
<li class="ed_txt">63X oil (SIM)</li>
<li class="ed_txt">100X oil (TIRF)</li>
</ul>
<div class="ed_txt"><strong>Laser wavelength (nm):</strong></div>
<ul>
<li class="ed_txt">405, 488, 561, 642</li>
</ul>
<div class="ed_txt"><strong>Observation Methods:</strong></div>
<ul>
<li class="ed_txt">Apotome</li>
<li class="ed_txt">Lattice SIM</li>
<li class="ed_txt">TIRF</li>
</ul>
<div class="ed_txt"><strong>Fluorescent Filters:</strong></div>
<ul>
<li class="ed_txt">Eyepiece observation：Filter set 77 HE GFP + mRFP + Alexa 633 shift free</li>
<li class="ed_txt">Image capture：
<ul>
<li class="ed_txt">Position 3: EF LBF 405/488/561/642</li>
<li class="ed_txt">Position 4: BP 420-480 / BP 495-550 / LP 655</li>
<li class="ed_txt">Position 5: EF BP 495-550/BP 570-620</li>
<li class="ed_txt">Position 6: BP 420-480/LP 655</li>
</ul>
</li>
</ul>
<div class="ed_txt"><strong>Camera:</strong></div>
<ul>
<li class="ed_txt">sCMOS</li>
</ul>
<div class="ed_txt"><strong>Live-cell Incubation System:</strong></div>
<ul>
<li class="ed_txt">Full hood and small stage-top incubator with temperature, humidity and CO<sub>2</sub> control modules</li>
<li class="ed_txt">The small living cell incubation system is erected on the stage, and the stable control concentration range of CO<sub>2</sub> is 0.0% to 10.0% (±0.1%), suitable for 35mm culture dishes with NO.1.5 (0.17mm) glass coverslips on the bottom.</li>
<li class="ed_txt">If you want to use the living cell system, please call the administrator before making your first appointment.</li>
<li class="ed_txt">With Definite Focus 2 auto focus maintaining system</li>
</ul>
<div class="ed_txt"><strong>Software:</strong></div>
<ul>
<li class="ed_txt">Zen</li>
<li class="ed_txt">Super-resolution module: SIM, SIM<sup>2</sup> module</li>
<li class="ed_txt">Apotome</li>
<li class="ed_txt">Offline computer workstation is available for image processing and analysis</li>
</ul>
</div>Sample Preparation<div class="ed_model01 clearfix">
<ol>
<li class="ed_txt"><strong>Coverslip</strong>
<ul>
<li class="ed_txt">We recommend using <strong>high-precision cover glasses with<span style="color:#ff0000;"> thickness no. 1.5</span></strong><span style="color:#ff0000;"> </span>to prepare the samples.
<ul>
<li class="ed_txt">Zeiss 474030-9010-000 Deckgläser 1,5H hi 100002240928 QS; Thickness no 1 ½ , high-performance 18 mm x 18 mm, 0.170 +/- 0,005 mm<br />
<img alt="Usage specifications" src="/userfiles/ordch/images/20231012152616164.png" /></li>
<li class="ed_txt">Marienfeld Superior: www.marienfeld-superior.com
<ul>
<li class="ed_txt">18×18 mm (Cat.No. 0107032).22×22 mm (Cat.No. 0107052).</li>
<li class="ed_txt">round with 18 mm diameter (Cat.No. 0117580).</li>
</ul>
</li>
</ul>
</li>
<li class="ed_txt">Fix your cells according to your preferred protocol.</li>
<li class="ed_txt"><strong>Please make sure that the <span style="color:#ff0000;">cover slip is centered on the glass slide</span></strong>, in order to fit in our samples holder.</li>
<li class="ed_txt">For glass-bottom petri dishes and multi-well plates see:
<ul>
<li class="ed_txt"><a href="http://www.willcowells.com/" rel="noreferrer noopener" target="_blank" title="Willco (Open New Windows)">Willco </a></li>
<li class="ed_txt">Lab-tek (offered through Thermo)</li>
<li class="ed_txt">MatTek (<a href="https://www.mattek.com/products/glass-bottom-dishes/" rel="noreferrer noopener" target="_blank" title="glass bottom dishes(Open New Windows)">glass bottom dishes</a>)</li>
<li class="ed_txt">ibidi µ-Dish 35 mm, high Glass Bottom (Cat.No:81158)</li>
</ul>
</li>
</ul>
</li>
<li class="ed_txt"><strong>Mounting Media</strong>
<ul>
<li class="ed_txt">Suggested Hardening media:
<ul>
<li class="ed_txt"><strong>ProLong™ Glass Antifade Mountant P36980 RI 1.52</strong></li>
<li class="ed_txt"><strong>ProLong™ Gold Antifade Mountant P36930 RI 1.46</strong></li>
</ul>
</li>
<li class="ed_txt">As it is a hardening media, you may also notice it can somewhat flatten samples.</li>
<li class="ed_txt">Do not use mounting media that include DAPI.</li>
<li class="ed_txt">Concerning the refractive index (RI) of the mounting medium, <span style="color:#ff0000;">prepare the slides at least 2 days before imaging</span>. Allowing the sample to have sufficient time for curing mounting media and sealant. (2–8°C)</li>
<li class="ed_txt">The following Aqueous(Non-Hardening) Mounting liquid media also works, despite having a lower refractive index. SlowFadeGold /Diamond RI of 1,42. VectaShield H-1000. RI of 1,44</li>
</ul>
</li>
<li class="ed_txt"><strong> Fluorescent Labels for SR-SIM</strong></li>
</ol>
<div class="ed_txt" style="margin-left: 40px;">All common types of organic dyes usually conjugated to antibodies or fluorescent proteins are suitable to be used for SR-SIM. <strong>For multicolor samples the fluorophores should be selected for minimal spectral overlap to avoid crosstalk.</strong></div>
<ul style="margin-left: 40px;">
<li class="ed_txt">405 nm (50 mW),</li>
<li class="ed_txt">488 nm (100 mW),</li>
<li class="ed_txt">561 nm (100 mW),</li>
<li class="ed_txt">642 nm (150 mW)</li>
</ul>
<div class="ed_txt" style="margin-left: 40px;">Note： <span style="color:#ff0000;">Cytosolic or other non-specific fluorescent protein expression (e.g. GFP) will result in staining of extended areas</span>. Since well-defined structures are missing to interfere with the grid pattern, modulation contrast will be low and the final image will lack high resolution information.</div>
</div>Applications<img alt="SIM2 " src="/userfiles/ordch/images/20241220170332746.png" /><br />
<img alt="SIM Apotome" src="/userfiles/ordch/images/20241220170332452.png" />Reservation Rules<div class="ed_model01 clearfix">
<div class="ed_txt"><span style="color:#e74c3c;"><span style="font-size:110%;"><strong>*Users must be certified.</strong></span></span><br />
<strong>Users must attend the Principles and Instruments training course, have confocal hands-on experience, and undergo hands-on instruction by the Specialist before applying for the certification test.</strong></div>
<ul>
<li class="ed_txt">Only those who have passed the certification examination and operated the device more than 5 times (inclusive) during office hours will be authorized to make a reservation.</li>
<li class="ed_txt">Each person can make 4 reservations within two weeks, and each reservation can be made for 4 hours.</li>
<li class="ed_txt"><strong>Reservation regulations</strong>:
<ul>
<li class="ed_txt"><strong>Please fill out the <a href="http://forms.gle/k1hr3wtmjj8G85am8" title="sample information Questionnaire">sample information Questionnaire</a> for first-time users and wait for the specialist's reply to confirm the time. </strong></li>
<li class="ed_txt"><strong>Sample testing: </strong>Each laboratory is limited to one reservation for the same type of samples (<strong>up to two hours of testing/teaching credit</strong>), and repeated reservations will be charged according to the time of use!</li>
<li class="ed_txt">Please go to the <a href="https://ircbooking.nycu.edu.tw/IRC/irc_booking/index_en.aspx" title=""Instrument Reservation System"">"Instrument Reservation System"</a> to make a reservation before use. The person who makes the reservation must be the actual user.</li>
<li class="ed_txt">After use, please register in the record book. The start time is the reservation time, and the end time is based on the end time of use. You cannot delay the start time or occupy other people's time slots.</li>
<li class="ed_txt">Reservations cannot be canceled on the same day. Reservations made more than half an hour after the scheduled time are considered abandoned.</li>
<li class="ed_txt">No same-day reservations are accepted. Please confirm your reservation by 16:30 of the previous workday at the latest; late changes violate the rules and will not be accepted</li>
</ul>
</li>
</ul>
</div>Opening Hours<ul>
<li class="ed_txt">Monday to Friday (Office Hours)
<ul>
<li class="ed_txt">08:30-17:00 Certified Users Only</li>
</ul>
</li>
<li class="ed_txt">weekend
<ul>
<li class="ed_txt">Temporarily closed</li>
</ul>
</li>
</ul>Charges<div class="table01">
<table class="ed_table caption-top" style="width:1200px;" summary="Instrument Charges">
<caption>Instrument Charges (NTD per hour)</caption>
<tbody>
<tr>
<th scope="col">Advanced optical microscope</th>
<th scope="col">Super-Resolution Microscope<br />
with Lattice SIM² and Incubation System</th>
</tr>
<tr>
<td class="ed_pc_center" data-th="special optical microscope">Model</td>
<td data-th="Super-resolution imaging system with living cell culture device" style="text-align: center;">(Z) Elyra 7</td>
</tr>
<tr>
<td class="ed_pc_center" data-th="special optical microscope">Fees for NYCU faculty and students</td>
<td data-th="Super-resolution imaging system with living cell culture device" style="text-align: center;">1,300</td>
</tr>
<tr>
<td class="ed_pc_center" data-th="special optical microscope">Fees for cooperative ducational unit</td>
<td data-th="Super-resolution imaging system with living cell culture device" style="text-align: center;">1,950</td>
</tr>
<tr>
<td class="ed_pc_center" data-th="special optical microscope">Fees for other unit</td>
<td data-th="Super-resolution imaging system with living cell culture device" style="text-align: center;">2,600</td>
</tr>
<tr>
<td class="ed_pc_center" data-th="special optical microscope">Remarks</td>
<td data-th="Super-resolution imaging system with living cell culture device">
<ol>
<li>Brand abbreviation: (Z) Zeiss</li>
<li><strong>Number of Reservations: Each person can make four reservations per device within a two-week period, with a maximum of four hours per reservation. Reservations can be made consecutively on all devices.</strong></li>
<li>Besides the imaging core facility education and training organized by the center, it is recommended to bring your samples for scheduled teaching and certification tests (experimental data can be taken away), and the charges will be the same as for general computer use.</li>
<li><strong>The above table shows the hourly usage charge. Charges are calculated on a half-hour basis.</strong></li>
</ol>
</td>
</tr>
</tbody>
</table>
</div>
<div class="ed_model01 clearfix">
<div class="ed_txt">If you have the following qualifications, you will be charged the same fee as <strong>NYCU faculty and students</strong>:</div>
<ul>
<li class="ed_txt">Branch school</li>
<li class="ed_txt">Joint-appointed faculty </li>
<li class="ed_txt">Affiliated hospitals </li>
<li class="ed_txt">Projects in cooperation with and under the control of the University.</li>
</ul>
<div class="ed_txt">If you have the following qualifications, the fee will be the same as that of a <strong>cooperative educational unit</strong>:</div>
<ul>
<li class="ed_txt">Adjunct faculty</li>
<li class="ed_txt">Teaching hospitals (Veterans General Hospital, Taipei City Hospital, Far Eastern Memorial Hospital, etc.)</li>
<li class="ed_txt">University System of Taiwan (UST)</li>
<li class="ed_txt">Companies participate in the academia-industry cooperation center of NYCU</li>
</ul>
<div class="ed_txt"><strong>Instruments are charged per half hour, and the billing will be counted at the end of each odd-numbered month. </strong>Please bring the bill to the Cashier's Section of the Administration Building within two weeks at the latest. The service will be stopped if the number of overdue payments reaches (and includes) two times.</div>
</div>Precautions<!-- 注意事項 -->
<div class="ed_model01 clearfix">
<ol>
<li class="ed_txt"><strong><span style="color:#e74c3c;">No USB flash drive is allowed. </span></strong>Please use FTP to transfer files or bring your blank CD-ROM to burn data. Due to the limited space of the computer's hard disk, the administrator will delete the data from time to time to facilitate the normal operation of the instrument. <span style="color:#e74c3c;">We are not responsible for data storage.</span></li>
<li class="ed_txt"><strong>Without the consent of the administrator of the Center, you are not allowed to add or remove any parts, alter the system settings, or install software on the Center's public equipment. </strong>If any experiment requires any test, the administrator's consent must be sought first and accompanied by the engineer of the original manufacturer to carry out the testing without damaging the normal operation of the equipment, and only after the passing of the test can it be operated and used by the personnel approved by the administrator of the Center under the condition of the administrator's permission.</li>
<li class="ed_txt">According to the administrator's judgment, the violator is warned for the first time, canceled the right to use for one month for the second time, and canceled the right to use permanently for the third time; if the violator violates the above specification, resulting in the damage of the instrument, the violator shall pay compensation to repair the instrument and stop the right to use for one month. The violator is prohibited from using the instrument permanently.</li>
</ol>
</div>Manuals & Videos<div class="ed_model17 clearfix ed_list">
<ul>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Introduction : Product info_Elyra-7</span> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231012153808178.pdf" rel="noreferrer noopener" target="_blank" title="EN_product-info_Elyra-7(Open New Windows)"><img alt="EN_product-info_Elyra-7" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><strong><span style="vertical-align: inherit;">Operation Manual : Quick Guide (20230314 version)</span></strong> </span>
<ul class="d-flex">
<li><a href="/userfiles/ordch/files/20231012153750207.pdf" rel="noreferrer noopener" target="_blank" title="Manual (20230314 version)(Open New Windows)"><img alt="Manual (20230314 version)" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><strong><span style="vertical-align: inherit;">Principle and Instrument Introduction : The New Era of Super-resolution Microscopy, Elyra 7</span></strong> </span>
<ul class="d-flex">
<li><a href="https://youtu.be/g2L9bJmY-E4?si=llHEoYBUW6c38SPf" rel="noreferrer noopener" target="_blank" title="The New Era of Super-resolution Microscopy, Elyra 7(Open New Windows)"><img alt="The New Era of Super-resolution Microscopy, Elyra 7" src="/userfiles/file/link.png" /> </a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;"><span style="vertical-align: inherit;">Elyra 7 Certification Application form</span></span>
<ul class="d-flex">
<li><a href="/userfiles/orden/files/20240229114211309.pdf" target="_blank" title="Certification Application(Open New Windows)"><img alt="Certification Application" src="/userfiles/file/pdf.png" /> </a></li>
</ul>
</li>
<li class="d-flex" style="text-align: justify;">User Rules for Confocal Microscopes and Advanced Optical Microscopes
<ul class="d-flex">
<li><a href="https://www.nycu.edu.tw/ord/en/app/artwebsite/doc?module=artwebsite&detailNo=1204339180721672192&type=s" target="_blank" title="User rules(Open New Windows)"><img alt="User rules" src="/userfiles/file/pdf.png" /></a></li>
</ul>
</li>
<li class="d-flex" style="text-align: justify;">ZEN lite download
<ul class="d-flex">
<li><a href="https://www.nycu.edu.tw/userfiles/ordch/files/Zen%20lite%20download.pdf" target="_blank" title="download instruction(Open New Windows)"><img alt="download instruction" src="/userfiles/file/pdf.png" /></a></li>
</ul>
</li>
<li class="d-flex"><span style="vertical-align: inherit;">ZEISS Knowledge Base</span>
<ul class="d-flex">
<li><a href="https://knowledge.zeiss.com/rms" rel="noreferrer noopener" target="_blank" title="Your knowledge base for ZEISS software(Open New Windows)"><img alt="Your knowledge base for ZEISS software" src="/userfiles/file/link.png" /> </a></li>
</ul>
</li>
</ul>
</div>https://ord.nycu.edu.tw/ord/en/app/machine/image?module=machine&detailNo=1164487948175937536&init=YZeiss Elyra 7https://www.youtube.com/embed/g2L9bJmY-E4youtubehttps://ord.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2023&serno=da69be39-dc4e-48d6-9168-7c96b2dbdb6eInstrument List of YM Campushttps://ord.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2024&serno=df4f722b-8c61-4ccb-92d3-1135d8d1bfe2Imaging Core of YM Campushttps://ord.nycu.edu.tw/ord/en/app/folder/2031Other Light Microscopes