{"subject":"[YangMing] Cell Sorter - Beckman CytoFLEX SRT","detailContent":"
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\"Beckman
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","dataClassName":null,"pubUnitName":"Instrumentation Resource Center ","posterDate":null,"updateDate":"2025-01-21","liaisonper":"Flow Cytometry Core Facility","liaisontel":"66473","liaisonfax":null,"liaisonemail":null,"languageurl":null,"page1Title":"Instrument Specifications","page1":"
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","page2Title":"Reservation and Usage Rules","page2":"
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Reservation method:
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","page3Title":"Opening Hours","page3":"","page4Title":"Charges","page4":"\r\n\r\n
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Charging standards (Unit: NTD)
Flow CytometryServicesUnitNYCU faculty and studentsCooperative educational unitsOther units
Cell Sorter:\r\n\t\t\t
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  • BD FACS Melody
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  • Beckman CytoFLEX SRT
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\r\n\t\t\tSelf-operation		per half hour300 450
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Technician operationper half hour400600800
An additional startup fee will be charged for technician operationper case1,0001,5002,000
Cell AnalyzerBD FACS Caliburper half hour210315420
Beckman CytoFLEX
\r\n\t\t\t(Self-operation)		per half hour140210280
Beckman CytoFLEX
\r\n\t\t\t(Technician operation)		per tube200300400
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","page5Title":"Precautions","page5":"\r\n
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  1. No USB flash drive is allowed. Please use FTP to transfer files or bring your blank CD-ROM to burn data. Due to the limited space of the computer's hard disk, the administrator will delete the data from time to time to facilitate the normal operation of the instrument. We are not responsible for data storage.
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  2. Without the consent of the administrator of the Center, you are not allowed to add or remove any parts, alter the system settings, or install software on the Center's public equipment. If any experiment requires any test, the administrator's consent must be sought first and accompanied by the engineer of the original manufacturer to carry out the testing without damaging the normal operation of the equipment, and only after the passing of the test can it be operated and used by the personnel approved by the administrator of the Center under the condition of the administrator's permission.
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  3. According to the administrator's judgment, the violator is warned for the first time, canceled the right to use for one month for the second time, and canceled the right to use permanently for the third time; if the violator violates the above specification, resulting in the damage of the instrument, the violator shall pay compensation to repair the instrument and stop the right to use for one month. The violator is prohibited from using the instrument permanently.
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","page6Title":"Cell concentration","page6":"
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Cell Concentration
NozzleCell typeCell concentration
100μmAll cells (Max. Size: 20μm)1 × 107/ml
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\r\nUsers can use different protocols according to their needs, such as antibody labeling or fluorescent gene transfection, but the following procedures must be completed before using the machine:\r\n
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  1. Samples must be filtered with a nylon mesh (mesh size: 45μm). It is recommended to use Falcon cell strainer #352235 tube with mesh or #352340 40μm mesh. Then, place the filtered cells in a Falcon 12 × 75mm tube (Cat#352052). The table above describes the cell concentration, and the minimum sorting volume is 1 mL. Note: Failure to filter the sample, resulting in nozzle blockage or machine damage, will result in liability for compensation. If the machine is clogged and cannot operate due to unfiltered cells, the center reserves the right to cancel the current appointment and charge usage fees.
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  2. If the cells need to be cultured after sorting, please prepare a collection tube containing a suitable cell culture medium and hand it over to the operator before the sorting service.
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  3. Note that providing unstained cells as control group samples is required for instrument calibration before sorting.
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  4. If you perform sorting with multiple fluorescent staining, you must provide single stained samples of each fluorescent marker for instrument adjustment and correction before sorting.
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","page7Title":null,"page7":null,"page8Title":null,"page8":null,"docs":[],"images":[{"fileurl":"https://ord.nycu.edu.tw/ord/en/app/machine/image?module=machine&detailNo=1167357148200439808&init=Y","expFile":"Beckman CytoFLEX SRT"}],"videos":[],"audios":[],"resources":[{"relateURL":"https://ord.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2023&serno=da69be39-dc4e-48d6-9168-7c96b2dbdb6e","relateName":"Instrument List of YM Campus"},{"relateURL":"https://ord.edu.tw/ord/en/app/artwebsite/view?module=artwebsite&id=2025&serno=53f9f473-c892-4997-81fd-fe0d482719f2","relateName":"Flow cytometry core of YM Campus"},{"relateURL":"https://www.nycu.edu.tw/ord/en/app/machine/list?module=machine&id=2049","relateName":"Other Cell Sorters"}]}