Fluorescence Microscopes
中文- Update Date：2025-03-11
- Units：Instrumentation Resource Center
[Boai] Multi-functional Integrated Single-Molecule Fluorescence Microscope and Spectrofluorometer
- Total Internal Reflection Fluorescence Inverted Microscopy System
- Abbreviation：MFI-SMFM
- Brand Model:
- Multi-functional Integrated Single-Molecule Fluorescence Microscope
- Model: Home-built microscopy
- Spectrofluorometer
- HORIBA
- Multi-functional Integrated Single-Molecule Fluorescence Microscope
- Instrument expert: Prof. Hsiao, Yu-Yuan
- ext 56999
- mike0617@nycu.edu.tw
- Instrument operation technician: Huang, Guan-Wei [full time]
- ext 56903
- alwaysthere.bt08@nycu.edu.tw
- Instrument location: Instrument Location: Room 433, Hsien Chee Bio-ICT Building
1. Multi-functional Integrated Single-Molecule Fluorescence Microscope
Instrument brand, model, year of purchase
- Brand: Home-built microscopy
- Model: Spectrum
- EMCCD: Andor IXON-L-888
- APD: Excelitas SPCM-AQRH-14
- Laser: Coherent Sapphire 532-300 CW
- Piezo stage: Physik Instrumente P-517.3CL
- Microscope Objective: Olympus IX73 UPLAPO100XOHR
- Purchase date: 2021
Important Specifications
- Excitation laser source: Sapphire 532nm / 300mW CW, Coherent (Non-polarized laser)
- Emission Fluorescence: 582nm±32、720nm±75
- EMCCD: iXon Life 888
- Pixel size 1024 x 1024
- Minimum temperature - 65ºC (30 MHz) - 80ºC(10 MHz)
- Digitization 16-bit (at all speeds)
- Detector: Avalanche Photodiode(APD)
- Dark Count Rate 100cps
- Max Count Rate 37Mcps
- Dead Time 22ns
- Instrumentation Control Software：GeneralPurposeSOS
- https://github.com/PrincetonUniversity/GeneralPurposeSOS
- The instrument setup references J. Chem. Phys. 148, 123316 (2018)
2. Spectrofluorometer
Instrument brand, model, year of purchase
- Brand: HORIBA
- Model: FluoroMax-4 PLUS
- Purchase date: 2020
Important Specifications
- Gratings: 1200 groove/mm blazed at 330 nm (excitation) and 500 nm (emission), plane ruled
- Detectors: Emission: R928P photon counting PMT (185-850 nm) and reference photodiode for monitoring lamp output
- Bandpass: 0 to 30 nm, continuously adjustable
- Accuracy: 0.5 nm
- Minimum step: 0.0525 nm
- Integration time: 0.001 to 160 sec
Services
1. Multi-functional Integrated Single-Molecule Fluorescence Microscope
- Wide-field TIRF imaging mode
- TIRF-coupled-Confocal smFRET acquisition mode
2. Spectrofluorometer
- Fluorescence spectrum scan
- Bulk fluorescence measurements
Opening hours
- Monday and Tuesday 9:00-17:00
- Other times (including Saturdays and Sundays): Closed
System Open Level
1. General working hours:
1. General working hours:
- Level B: Spectrofluorometer
- Level D: Multi-functional Integrated Single-Molecule Fluorescence Microscope
2. Night and holiday periods: Closed
【System Level Description】
【System Level Description】
- Level A: Open to students who need to use it. They can operate it on their own after training and assessment.
- Level B: Each professor nominates one student for training, while the rest operate under those who have been trained. Professors with multiple students using the instrument can request an increase in the number of students receiving training.
- Level C: The instrument administrator selects several students recommended by the professor to receive training. After passing the assessment, they can self-operate the instrument and be responsible for delegated operation service.
- Level D: The laboratory's technical staff only accept delegated services and are unavailable for general use.
Charging standards
1. Multi-functional Integrated Single-Molecule Fluorescence Microscope
1. Multi-functional Integrated Single-Molecule Fluorescence Microscope
- On-campus: NT$3000 / hour
- Off-campus: NT$9000 / hour
2. Spectrofluorometer
- On-campus: NT$500/ hour
- Off-campus: NT$1500 / hour
Note:
- Academic units refer to colleges and universities.
- Please prepare the necessary consumables by yourself.
- Instrument usage will be charged for a minimum of one hour, even if the actual operational time is less than an hour.
Instrument usage regulations
- Before booking this instrument, please discuss with the instrument manager whether your experimental samples and design meet the instrument's measurement range. This includes, but is not limited to, informing the manager about:
- Sample type (coverslips or other materials)
- Fluorescent dye (excitation/emission) wavelengths
- Observation of infectious or isotope samples is strictly prohibited.
- Recommended coverslip size: 35 x 35mm or larger, thickness 0.13-0.19mm.
- Before using the MTI-SMFM, please provide fluorescence spectroscopy experimental data and discuss with the instrument manager to confirm compatibility. If no relevant data is available, please book the fluorescence spectrometer in this facility for testing.
- Operate the instrument according to standard procedures. Comply with instrument usage rules.
- After continuous operation for 4 hours, the instrument must rest for at least 1 hour.
- Keep the instrument clean after use. Complete the usage registration form and notify the instrument manager for on-site inspection.
- In case of instrument malfunction:
- Immediately inform technical personnel
- Do not disassemble the instrument
- Quickly report failure details to the technical operator or manager
- Personal-caused damage will be repaired at the user's expense
- USB data access is strictly prohibited. Violation will result in usage rights suspension. Do not store data on the computer. Bring your own DVD for data storage.
- If a paper is published using this instrument, one usage fee (2 hours) will be waived.
Instrument Management
- The instrument is managed by the faculty members from the Institute of Molecular Medicine & Bioengineering, who will collectively discuss management personnel, fee standards, and usage matters.
- Educational training for the instrument will be announced and implemented only after recommended users are endorsed by a professor. Training may involve a nominal processing fee if necessary.
- One instrument manager is appointed to oversee equipment management and peripheral environment maintenance. Future additional personnel will be determined based on usage conditions.
- Instrument billing is time-based, with reservation time as the starting point. Reservation must be made one week in advance. Cancellations must be made at least three days before the scheduled use date, otherwise full reservation time will be charged.
- No additional components may be installed, system settings altered, or software installed without the instrument manager's explicit consent.
- Users must verify system normality before each use, accurately log usage in the registration book, and restore the instrument to its original state. Any issues should be immediately reported to the instrument manager to avoid usage disputes and clarify responsibilities.
- Violation of these regulations may result in the manager suspending the user's usage rights. If improper use causes instrument damage, the responsible laboratory head will be liable for related compensation.
Term of use:
- Users are required to pass certification to use it
- Participate in this "Equipment Usage Principles" and "Equipment Hands-on Training" courses and pass the assessment to obtain a certification to use it yourself.
Instrument Reservation:
- Users MUST make reservations one week before and submit the application form before use.
- After the reservation is confirmed, you must notify the cancellation three days before use if you cannot use it for any reason. If you do not cancel three days in advance, you will be regarded as using the reservation period and still have to pay.
