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Fluorescence Microscopes

exchangealt 中文
  • Update Date：2025-02-25
  • Units：Instrumentation Resource Center
[Boai] Total Internal Reflection Fluorescence Inverted Microscope - Nikon Ti-TIRF-E
Total reflection fluorescence inverted microscope system
  • Total Internal Reflection Fluorescence Inverted Microscopy System
    • Abbreviation：TIRF
    • Brand Model: Nikon Ti-TIRF-E
  • Instrument expert: Prof. Huang, Eric
    • ext 56968
  • Instrument operation technician: Lai, Yu-Hsuan
    • ext 56969
  • Contact Person: Miss Lai Siyi
    • ext 56941
  • Instrument location: Room 620, Hsien Chee Bio-ICT Building
Instrument brand, model, year of purchase
Brand: Nikon
Model: Nikon Ti-TIRF-E
Purchase: December, 2011

Specifications
  1. Fully automatic inverted fluorescence microscope (Nikon Ti-E)
  2. Laser system: 488 nm solid-state laser system + 561nm solid-state laser system
  3. Fully automatic total reflection fluorescence system (Nikon Ti-TIRF-E)
  4. In vivo microscopy system (Tokai Hit INU-TIZHB-F1)
Services
  1. Total internal reflection fluorescence imaging
  2. Live cell imaging
  3. Multi-color fluorescence imaging
  4. Automatic multi-position acquisition
  5. Other fluorescence image analysis
Opening hours
  1. Monday to Friday
    • Morning: 08:30~12:30
    • Afternoon: 13:30~17:30
    • Night: 18:00~22:00
  2. Currently closed on Saturdays and Sundays
System opening level
  1. Working hours: Class A
  2. Night time period: Class B
Note:
  • Level A: Open to students who need to use it. They can operate it independently after passing education training and instrument administrator certification.
  • Level B: Only those with a Level A  license and who have operated more than ten times can apply for a Level B license training and certification.
  • On campus
    • NT$1,000/hour during working hours (08:30~17:30)
    • NT$2,000/hour during non-working hours (18:00~22:00)
  • Outside campus
    • NT$2,000/hour during working hours (08:30~17:30)
    • NT$4,000/hour during non-working hours (18:00~22:00)
Usage Rules for Total Internal Reflection Fluorescence Inverted Microscope:
  1. To use this equipment, you must make a reservation in advance via the Faces Scheduling System (http://faces.ccrc.uga.edu/) and confirm it by the administrator before use.
  2. For first-time use, please contact the administrator (ext. 56994) to apply for a user account before logging into the system for reservation.
  3. The management unit will organize at least one yearly seminar to provide educational training and on-site examinations. It will charge a fee for each type of training if necessary.
  4. A technician (assistant instructor for instrument operation) will be assigned to manage and maintain the equipment. Under the guidance of the management committee, the equipment and its surroundings will be managed, and technical support will be provided.
  5. General or preliminary observations should not be performed on the equipment to avoid time-consuming occupation. The use of isotopes and infectious samples is prohibited.
  6. Users must be educated and trained (including hands-on training) before using the instrument according to the regulations. In addition to doubling the charges, the right to use the instrument will also be terminated if there are cases of fraudulent use of other people's accounts without certification.
  7. If a reservation has not been canceled three days in advance, you will still be charged for the reserved time slot.
  8. If the machine is damaged by improper use, the person in charge of the lab will compensate for the loss.
  9. To maintain and improve the quality of service, those who use the images captured by this instrument in their master's thesis or journal publications should mention the instrument's name and the organization in the acknowledgment or methodology of the thesis.
Instructions for using the total internal reflection fluorescence inverted microscope system:
  1. Applicants who have attended the course on the principles of the Total Internal Reflective Fluorescent Inverted Microscope (TIRF) and the hands-on training course and have been certified by the instrument administrator are authorized to make a reservation for using TIRF.
  2. The fee for the TIRF inverted microscope is charged on a time basis, with the reservation time as the starting point. Reservations must be made one week in advance and canceled three days before the use date. Otherwise, the fee will still be charged according to the reserved time.
  3. Charges are calculated from the reserved time, and every 10 minutes or more will be counted as one hour, and delays caused by the previous user will not be counted.
  4. If you do not show up 30 minutes after the scheduled time, the time slot may be assigned to another person who requires it. The latter lab will be charged for the use of the machine.
  5. No parts may be added, system settings changed, or software installed without the approval of the instrument specialist and system engineer.
  6. Applicants are required to inform the instrument administrator at the time of booking of the type of samples to be used (slides, dishes, or other materials), the type of cells or animal tissues to be used, and the fluorescent (excitation/scattering) spectrum of dyes to be used. Infectious and isotopic samples are not permitted to be observed on the instrument.
  7. Users should check whether the system is working properly each time they use the system and register the system in the User Register after use. If there is any problem, they should notify the instrument administrator immediately to avoid disputes over the instrument's use and clarify the responsibility.
  8. After use, the user shall be responsible for returning the instrument to its original condition, and if any oil lens is used, it shall be cleaned, and the microscope system shall be kept clean.
  9. Data can only be accessed using a brand new (DVD/VCD) CD-ROM and cannot be stored in any other way.
  10. In case of violation of the above regulations, the administrator may suspend the right of use according to the circumstances, and the person in charge of the laboratory shall be responsible for compensation if the equipment is damaged due to human misuse.
  11. The applicant must fill out a statement to prove they understand the rules and regulations when using the instrument for the first time.
  • TIRF microscopy system use statement
Instrument training operation application instructions
Qualification for use:
  • To use this instrument, you must attend the "TIRF Principles of Operation" and "TIRF Hands-on Training" courses and be certified by the instrument administrator before obtaining your authorization.
  • You must fill in the statement the first time you use the instrument.
  • Note: At least one workshop will be held annually for educational training, and the cost of various trainings will be charged if necessary.
Instrument Operation Reservation:
  1. You must reserve the instrument one week before the scheduled use date and submit the statement (for the first time).
  2. If you cannot use it for any reason after the reservation is confirmed, you must inform us in advance three days before the use date. If you do not cancel the reservation three days in advance, it will be regarded as the same as using the reserved time slot, and you will still have to pay the fee.
  3. Charges will be calculated from the reservation time and counted as one hour for every 10 minutes or more of the reserved time. The delay caused by the previous user will not be calculated.
  4. If you do not show up 30 minutes after the scheduled time, the time slot may be assigned to another person who requires the service.
  5. The minimum usage time of the equipment is two hours.
Instrument List of Guangfu anf Boai Campus Other Fluorescence Microscopes
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