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Confocal Microscopes

exchangealt 中文
  • Update Date：2024-06-27
  • Units：Instrumentation Resource Center
[YangMing] Inverted Laser Scanning Confocal Microscope - Olympus FV1000
Laser Scanning Conjugate Focus Microscopy Olympus FV1000
  • Inverted Laser Scanning Confocal Microscope
  • Instrument Location: Room B03, B1 Floor, Shou-ren Building
  • Person in charge: YM Campus Imaging Core Facility
  • Term of use: Certification required. 
    • Only those who have passed the certification test after the instruction and have operated the device more than 5 times during office hours will be authorized to make a reservation.
  • Charges: NT$500 per hour for NYCU users. Please refer to the charging standard for off-campus use.
  • Reservation >>> Instrument Reservation System
Microscope:
  • Inverted microscope Olympus IX-81
Objectives:
  • 10X, 20X, 40X (Note: This 40X is not an oil lens.)
  • 60X Oil, 100X Oil
Laser wavelength (nm):
  • Diode Laser: 405nm, 440nm, 405nm SIM laser (for photobleaching)
  • Argon Laser: (458/488/515nm)
  • HeNe Laser: 543nm, 633nm
Detectors:
  • 3 PMT, can acquire three channels simultaneously (four channels if including DIC).
  • Spectral type, Emmision bandwidth can be adjusted according to the needs.
  • T-PMT, for simultaneous acquisition of transmitted light Differential Interferometry DIC images.
Live-cell Incubation: none

Software:
  • Photobleach, (FRET, FRAP, FLIP, photoactivate, etc.)
  • Tornado scanning
  • Timelapse imaging
  • Data analysis
  • Unmixing and Lambda scan
  • Colocalization
  • Multi-channel scan
  • 3D stereo image reconstruction Z-axis scan
*Users must be certified.
Users should pass the certification test after the instruction and operate the system more than five times (inclusive) during office hours (8:30-16:30) before they are authorized to reserve online, have access to Room B03 and can use the system according to the regulations. If the certified user does not use the device for one month, they must be certified again by the administrator.
  1. Reservation regulations:
    • Please go to the "Instrument Reservation System" to make a reservation before use. The person who makes the reservation must be the actual user.
    • After use, please register in the record book. The start time is the reservation time, and the end time is based on the end time of use. You cannot delay the start time or occupy other people's sessions.
    • Reservations cannot be canceled on the same day. Reservations made more than half an hour after the scheduled time are considered abandoned.
    • No same-day reservations are accepted. Please confirm your reservation by 16:30 of the previous workday at the latest; late changes violate the rules and will not be accepted.
    • Each person can make 4 reservations within two weeks, and each reservation can be made for 2 hours.
  2. Sample requirements:
    • Always use 0.17mm ( No.1.5) glass coverslips for slide samples or glass-bottom Petri dishes.
    • Please be sure to clean the surface of your samples before loading.
    • Please ensure the samples are sealed and a fluorescent microscope is used to confirm the samples are fluorescent before loading. If the samples cannot be serviced due to their own factors, the center will still charge according to the hours of operation.
    • If you have special needs, please inform us of the dyes used for the samples before operations so the technician can understand and advise the user's needs.
    • Please contact the center administrator for any other needs or special requirements.
  • Monday to Friday
    • full time　00:00-24:00　Certified users can make online reservations
  • weekend
    • full time　00:00-24:00　Not open for use
 　　 　　 　　 　　 　　
Instrument Charges (NTD per Hour)
Confocal
Microscope		 (Z) LSM880 (Z) LSM900 (O) FV1000
(Z) LSM700		 (O) FV10i (Z) LSM7MP
  Office hours at night Living cells Fluorescence Office hours at night Living cells Fluorescence      
Restricted to Certified Users   V V   V V V V V
Fees for NYCU faculty and students 900 500 300 800 600 300 500 300 1,000
Fees for cooperative educational units 1,350 -- 450 1,200 -- 450 750 450 1,500
Fees for other unit 1,800 -- 600 1,600 -- 600 1,000 600 2,000
Remarks
  1. Abbreviations: (O) Olympus, (Z) Zeiss
  2. Number of Reservations: Each person can make up to four reservations on a single device within a two-week period. Nighttime use and LSM7MP allow up to 3 hours per reservation, while other devices allow up to 2 hours per reservation. Reservations can be made consecutively on all devices.
  3. The office hours are from 08:30 to 16:30, and the nighttime period is from 16:30 to 08:30 of the next day.
  4. If you need to take time-lapse imaging overnight, you must contact the administrator of the center in advance.
  5. LSM 7MP is open from 09:00 to 17:00. If you need to operate at night, you must inform the administrator in advance.
  6. In addition to the education and training of the imaging core facilities organized by the center, it is recommended that you prepare your own samples (experimental data can be taken away) for scheduled training and certification tests. The charges will be the same as for general use of the system.
  7. The above table shows the hourly usage charge. Charges are calculated on a half-hour basis.
  1. If you have the following qualifications, you will be charged the same fee as NYCU faculty and students:
    • Branch school
    • Joint-appointed faculty 
    • Affiliated hospitals 
    • Projects are done in cooperation with and under the control of the university.
  2. If you have the following qualifications, the fee will be the same as that of a cooperative educational unit:
    • Adjunct faculty
    • Teaching hospitals (Veterans General Hospital, Taipei City Hospital, Far Eastern Memorial Hospital, etc.)
    • University System of Taiwan (UST)
    • Companies participate in the academia-industry cooperation center of NYCU
Instruments are charged per half hour, and the billing will be counted at the end of each odd-numbered month. Please bring the bill to the Cashier's Section of the Administration Building within two weeks at the latest. The service will be stopped if the number of overdue payments reaches (and includes) two times.
  1. No USB flash drive is allowed. Please use FTP to transfer files or bring your blank CD-ROM to burn data. Due to the limited space of the computer's hard disk, the administrator will delete the data from time to time to facilitate the normal operation of the instrument. We are not responsible for data storage.
  2. Without the consent of the administrator of the Center, you are not allowed to add or remove any parts, alter the system settings, or install software on the Center's public equipment. If any experiment requires any test, the administrator's consent must be sought first and accompanied by the engineer of the original manufacturer to carry out the testing without damaging the normal operation of the equipment, and only after the passing of the test can it be operated and used by the personnel approved by the administrator of the Center under the condition of the administrator's permission.
  3. According to the administrator's judgment, the violator is warned for the first time, canceled the right to use for one month for the second time, and canceled the right to use permanently for the third time; if the violator violates the above specification, resulting in the damage of the instrument, the violator shall pay compensation to repair the instrument and stop the right to use for one month. The violator is prohibited from using the instrument permanently.
  • FV1000 Operation Manual 
  • Certification form-confocal_2022
  • User Rules for Confocal Microscopes and Advanced Optical Microscopes
    • User rules
Instrument List of YM Campus Imaging Core of YM Campus Other Confocal Microscopes
Related Image(s)：
  • Contact： [ ext. 66185]
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