Biomedical Imaging
- Update Date:2025-03-31
- Units:Instrumentation Resource Center
[Boai] IVIS Lumina S5 Imaging System

- IVIS Lumina S5 Imaging System
- Model: Revvity IVIS Lumina S5
- Instrument expert:
- Prof. Chao, Jui-I
- Extension 56965
- Prof. Chao, Chi-Hong
- Extension 59725
- Prof. Chao, Jui-I
- Instrument Operation Technician: Tong, Xiang-Ting [full time]
- Extension 56963
- E-mail japs6419st@gmail.com
- Contact Person: Ms. Lai Siyi
- Extension 56941
- Instrument Location: Animal Center Room202, Boai Campus
IVIS Lumina S5 Imaging System
Instrument brand, model, year of purchase
Instrument brand, model, year of purchase
- Brand: Revvity
- Model: IVIS Lumina S5
- Purchase date: November, 2024
Important Specifications
- IVIS Lumina S5 Imaging System
- PerkinElmer RAS-4 Rodent Anesthesia System
- Resolution 2048×2048
- Detection range 410nm-790nm (can be applied to various luminescence/fluorescent imaging)
- Field size 2.7x2.7 cm
- Heated lifting photography platform for small animals
- Fluorescent light source
- Fluorescence detection filter
- Excitation: 410-790 nm
- Emission: 500-865 nm
Services
- Absolute quantitative analysis and measurement of luminescence for living animals and plants
- Absolute quantitative analysis and measurement of fluorescence for living animals and plants
- Absolute quantitative analysis and measurement of luminescence cell lines
- Absolute quantitative analysis and measurement of luminescent strains
- Absolute quantitative analysis and measurement of fluorescent cell lines.
- Absolute quantitative analysis and measurement of fluorescent strains.
- Three-dimensional image reconstruction and signal depth detection
- Mouse animal organ model set and signal position analysis
- 3D image output
- Multi-fluorescence analysis and automatic background subtraction
Opening Hours
- Monday to Thursday: 9:00 – 17:00
- Friday: 9:00 – 1200
System Open Level
- General working hours: Level B
- Night and holiday periods: Level C
System Level Description
- Level A: Open to students who need to use it. They can operate it on their own after training and assessment.
- Level B: Each professor nominates one student for training, while the rest operate under those who have been trained. Professors with multiple students using the instrument can request an increase in the number of students receiving training.
- Level C: The instrument administrator selects several students recommended by the professor to receive training. After passing the assessment, they can self-operate the instrument and be responsible for delegated operation service.
- Level D: The laboratory's technical staff only accept delegated services and are unavailable for general use.
Charging standards
- On-campus: NT$1,200/hour (please prepare your own Isoflurane)
- Off-campus: NT$3,000/hour (please prepare your own Isoflurane)
Instrument usage regulations:
- This instrument is designed for general tissue or cell samples. If you intend to experiment with other infectious samples, please discuss it with the technical operator first.
- Wearing gloves while operating computers is strictly prohibited.
- The standard operating procedures must be followed while using the instrument. Please make sure to comply with the instrument usage regulations. Failure to follow the regulations three times will suspend the right to use.
- Please thoroughly clean the instrument and operating surface after use. Fill out the instrument usage registration form and notify the instrument administrator to conduct an inspection (clean with 70% alcohol).
- If the instrument malfunctions, please inform the technical personnel immediately. Do not attempt to disassemble or assemble the instrument yourself. Inform the technical operator or instrument manager of the instrument failure immediately. You must bear the related repair costs if the instrument is damaged for personal reasons.
- You can only use Isoflurane in gas anesthesia devices.
- To access image-related data, bring your own DVD burner. Saving files on the computer and USB access are strictly prohibited. Violators will have their right to use it suspended for one month.
- If you publish papers in journals using IVIS equipment, you can offset the one-time usage fee.
Instrument Management
- The instrument is managed by full-time faculty members with experience operating it in the College of Life Science. Relevant personnel, funding, and usage issues are discussed with full-time faculty members with experience operating the instrument from the College of Life Science.
- Personnel, funding, and charging standards must be submitted to the Department's Resource Committee for resolution and implemented after approval by the Department Affairs Meeting.
- At least one or two seminars are held annually for education and training. If necessary, various training fees may be charged.
- The instrument has an assistant for equipment management and maintaining the surrounding environment. Depending on usage conditions, it will be decided whether to increase manpower.
- The instrument has several instrument seed instructors. They are postgraduate students with certification and operating licenses (certified and issued by the instrument manufacturer) who can lead and assist users in operating the instrument. In principle, seed instructors can be paid with a work-study allowance. However, no salary will be paid if the seed instructor leads their own laboratory users to operate the instruments.
Term of use:
- Users are required to pass certification to use it
- Participate in the "IVIS Usage Principles" and "IVIS Hands-on Training" courses and pass the assessment to obtain a certification to use it yourself.
Note: This instrument holds at least one or two seminars annually for education and training. If necessary, various training fees may be charged.
Instrument Reservation:
- Users MUST make reservations one week before and submit the application form before use.
- After the reservation is confirmed, you must notify the cancellation three days before use if you cannot use it for any reason. If you do not cancel three days in advance, you will be regarded as using the reservation period and still have to pay.
Note:
- Charges will be calculated from the reservation time. Exceeding 10 minutes will be counted as one hour. If you are delayed due to the previous user's use, this will not be included in your reservation time.
- If you are 30 minutes late for your scheduled reservation, your time slot may be given to other users who need to use the machine. If you encounter a problem with the experiment and cannot use the machine, please cancel your reservation immediately. If you do not cancel, you will still be charged the full fee for your reservation period.
- If you need to cancel your reservation, please notify us at least three days in advance. You will not receive a refund if you cancel less than three days in advance. Please do not cancel without a valid reason, as this wastes manpower, time, medicine, and laser life.
- If you participate in regularly held training courses, you will not be charged a teaching fee. However, you will be charged a teaching fee if you request teaching outside of the teaching hours.
- After completing training and passing the assessment, you can operate and use the instrument independently. Please refer to the above charging standards.
- Our charging standards are recalculated every January and July. If adjustments are made, we will announce them separately.
Related Image(s):