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Spectrophotometer

exchangealt 中文
  • Update Date：2024-05-14
  • Units：Instrumentation Resource Center
[Boai] Integrated molecular spectroscopy system (ECD & FTIR)
UV/Near Infrared Circular Dichroism Spectrometer
  • Electronic Circular Dichroism Spectrometers (ECD) and Fourier-transform infrared spectroscopy (FTIR)
    • Brand/Model: JASCO, J-1700 (ECD); JASCO, FTIR-4600 (FTIR)
  • Instrument Expert: Prof. Li, Ming-Chia
    • Extension 59724
  • Instrument Operation Technician: Yang, Chi-Ming
  • Contact Person: Ms. Lai Siyi
    • Extension 56941
  • Instrument Location: Room 621, Hsien Chee Bio-ICT Building 
  1. Electronic Circular Dichroism Spectrometers (ECD)
    • Instrument brand, model, year of purchase
      • Brand: JASCO
      • Model: J-1700
      • Year of Purchase: 2015
    • Important Specifications
      • Light source: 450 W water-cooled Xenon lamp, 150 W Halogen lamp
      • Detector: Head-on photomultiplier tube PMT, InGaAs
      • Modulator: Piezoelastic modulator
      • Monochromator movable wavelength range: 163 to 2500 nm
      • Measurement wavelength range:
        • 163 to 950 nm (PMT detector)
        • 700 to 2500 nm (InGaAs detector)
      • Wavelength accuracy:
        • ±0.1 nm from 163 to 250 nm
        • ±0.2 nm from 250 to 500 nm
        • ±0.5 nm from 500 to 800 nm
        • ±1.5 nm from 800 to 1200 nm
        • ±2.0 nm from 1200 to 1600 nm
        • ±5.0 nm from 1600 to 2500nm
      • Wavelength repeatability:
        • ±0.05 nm from 163 to 500 nm
        • ±0.1 nm from 500 to 800 nm
        • ±0.5 nm from 800 to 1600 nm
        • ±1 nm from 1600 to 2500 nm
      • Spectral bandwidth:
        • 0.01 to 16 nm (~950nm)
        • 5, 10, 20, 30, 40, 50 nm (950～2500nm)
      • Slit width: 1 to 4000 mm
      • Digital Integration Time (D.I.T.): 0.1 msec to 30 sec.
      • Scanning mode: Continuous scan; Step scan; Auto response (D.I.T) scan
      • Scanning speed: up to 10000 nm/min
      • CD full scale: ±8000 mdeg
      • CD resolution: 0.00001 mdeg
      • Stray light: Not more than 0.0003% (200 nm)
      • RMS noise:
        • 0.003 mdeg (185 nm, 450 W light source), 0.007 mdeg (200 nm, 150/450 W light source), 0.007 mdeg (500 nm, 150/450 W light source)
        • (spectral bandwidth 1 nm, D.I.T. 8 sec)
      • Baseline stability: 0.02 mdeg/hr
      • LD measurement: Provided as standard, Full scale ±1 OD
      • UV measurement:    Provided as standard, Full scale up to 5 Abs
      • External input terminal: Two channels (input range: -1 to 1 V DC)
  2. Fourier-transform infrared spectroscopy (FTIR)
    • Instrument brand, model, year of purchase
      • Brand: JASCO
      • Model: FTIR-4600
      • Year of Purchase: October 2020
    • Important Specifications
      • Contains single reflection total reflection device Model: ATR PRO ONE ZnSe window crystal set
      • Including control computer X1
      • Spectral measurement wave number range: 7800 ~ 550㎝-1
      • Wave number accuracy: ±0.01㎝-1
      • Highest resolution: 0.7㎝-1
      • Gain (noise ratio S/N ratio) ≧ 25000: 1
      • Light source: high-intensity ceramic light source system
Services
  1. Electronic Circular Dichroism Spectrometers (ECD)
    • This device is perfect for protein structure analysis, enzyme reaction determination, and studying diseases caused by changes in protein configuration. It can also study chiral chromophores like glucose and lactic acid. J-1700 has a measurement wavelength range of 163nm to 2,500nm, covering ultraviolet, visible, and near-infrared areas, and can be used as a spectrometer to study tissue optics.
  2. Fourier-transform infrared spectroscopy (FTIR)
    • It can be used to analyze the transmission and reflection of infrared light, as well as the absorption spectra, chemical structure, and composition of both organic and inorganic materials.
Opening Hours
  1. Tuesday and Thursday: 9:00-12:00, 14:00-17:00
  2. Other times and Saturdays and Sundays: closed
System opening level
  1. General working hours: Level B
  2. Night and holiday hours: closed
Level Description
  • Level A: Open to students who need to use it. They can operate it on their own after training and assessment.
  • Level B: Each professor nominates one student for training, while the rest operate under those who have been trained. Professors with multiple students using the instrument can request an increase in the number of students receiving training.
  • Level C: The instrument administrator selects several students recommended by the professor to receive training. After passing the assessment, they can self-operate the instrument and be responsible for delegated operation service.
  • Level D: The laboratory's technical staff only accept delegated services and are unavailable for general use.
ECD and FTIR are charged according to the usage period:
  • Academic unit NT$3,000/time
  • Off-campus non-academic units NT$10,000/time
  • The unit of each use is three hours (less than three hours will be counted as three hours).
  • Remarks: Academic units refer to universities and colleges.
  • If two or more system items are reserved for a single experiment, the second item of the system can be charged at a discounted rate: NT$1,000 per hour for academic units and NT$3,000 per hour for Off-campus non-academic units.

（１）

Instrument Usage Regulations:
  1. Wearing gloves while operating computers is strictly prohibited.
  2. The standard operating procedures must be followed while using the instrument. Please make sure to comply with the instrument usage regulations. Failure to follow the regulations three times will suspend the right to use.
  3. Please thoroughly clean the instrument and operating surface after use. Fill out the instrument usage registration form and notify the instrument administrator to conduct an inspection.
  4. If the instrument malfunctions, please inform the technical personnel immediately. Do not attempt to disassemble or assemble the instrument yourself. Inform the technical operator or instrument manager of the instrument failure immediately. You must bear the related repair costs if the instrument is damaged for personal reasons.
  5. To access image-related data, bring your own DVD burner. Saving files on the computer and USB access are strictly prohibited. Violators will have their right to use it suspended for one month.
  6. If you publish papers in journals using this equipment, you can offset the one-time usage fee.

（２）

Instrument Management
  1. The instrument is managed by full-time faculty members with experience operating it in the College of Life Science. Relevant personnel, funding, and usage issues are discussed with full-time faculty members with experience operating the instrument from the College of Life Science.
  2. Personnel, funding, and charging standards must be submitted to the Department's Resource Committee for resolution and implemented after approval by the Department Affairs Meeting.
  3. At least one or two seminars are held annually for education and training. If necessary, various training fees may be charged.
  4. The instrument has an assistant for equipment management and maintaining the surrounding environment. Depending on usage conditions, it will be decided whether to increase manpower.
  5. The instrument has several instrument seed instructors. They are postgraduate students with certification and operating licenses (certified and issued by the instrument manufacturer) who can lead and assist users in operating the instrument. In principle, seed instructors can be paid with a work-study allowance. However, no salary will be paid if the seed instructor leads their own laboratory users to operate the instruments.
Instrument Training Application Instructions
Eligibility:
  1. Users require certification to use and operate this instrument.
  2. Users are required to participate in "principles of use" and "hands-on operation" courses of the detailed instrument, along with passing the certification test to obtain a self-operation license.
  3. Note that seminars are held for education and training, and training fees may apply if necessary.

Instrument Operation Reservation
Use reservation:
  1. Users must register a reservation one week before the scheduled date and submit the application form.
  2. Once your reservation is confirmed, you must notify us of any cancellations at least three days before the scheduled use. Failure to do so will result in the reservation period being considered as used, and you will still be required to make the payment.
Instrument List of Guanfu and Boai Campus Other Spectrometers
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