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NYCU Office of Research and Development

3D Printer

  • Update Date:2025-01-06
  • Units:Instrumentation Resource Center
[Boai] 3D Bioprinter
Temperature-controlled quantitative bioprinting system
  • regenHU 3D Discovery instrument 3DD-G05
    • Abbreviation: 3D bioprinter
    • Brand: regenHU
    • Model: 3D Discovery instrument 3DD-G05
  • Instrument Expert: Prof. Li, Ming-Chia
    • ext 59724
  • Instrument Operating Technician: Yang, Chi-Ming
  • Contact Person: Ms. Lai Siyi
    • ext 56941
  • Instrument Location: Room 621, Hsien Chee Bio-ICT Building
Instrument brand, model, purchase period
Brand: regenHU
Model: 3D Discovery instrument 3DD-G05
Purchase period: 2019

Important Specifications
  • regenHU 3D Discovery instrument 3DD-G05
    • Including operating computer
    • BioCAD drawing software
    • BioCAM drawing software
    • BioCUT drawing software
    • Correction system
  • DD-135N Pneumatic Extrusion Printing Head
    • Applicable to fluid samples with a viscosity of 50-200000mPa.s
  • CF-300H Cell Compatible Print Head
    • Suitable for fluid samples with a viscosity of 1-1000mPa.s, which can improve cell viability
  • CU-05 Temperature Controller (Water Bath)
    • Water bath temperature control 5-37℃
    • 3DD dedicated connection cable
    • The temperature control board and the barrel temperature control can be additionally connected
  • CH-03CC Barrel Controller
  • CF-300 3ml Barrel Thermostat
  • TB-05C 3DD Making Baseboard Thermostat
  • Making a Bun Thermostat
Service Items
To develop 3D-printed cytoskeletons that are both functional and biologically active and enable cell printing and cultivation in a temperature-controlled environment to maintain the biological activity of cells during the 3D printing process. The temperature for cell printing will be controlled within the range of 5-37°C.
 
Due to environmental considerations, the instrument is currently limited to printing general structures. However, we plan to evaluate its capabilities and make it available for printing samples containing biological cells.
 
Opening hours
  1. Tuesdays and Thursdays: 9:00-12:00, 14:00-17:00
  2. Other times (including Saturdays and Sundays): closed
System Open Level
  1. General working hours: Level B
  2. Night and Holiday Periods: Closed
Level Description
  • Level A: Open to students who need to use it. They can operate it on their own after training and assessment.
  • Level B: Each professor nominates one student for training, while the rest operate under those who have been trained. Professors with multiple students using the instrument can request an increase in the number of students receiving training.
  • Level C: The instrument administrator selects several students recommended by the professor to receive training. After passing the assessment, they can self-operate the instrument and be responsible for delegated operation service.
  • Level D: The laboratory's technical staff only accept delegated services and are unavailable for general use.
Charges
  • Charges based on usage period:
    • Academic unit: NT$10,000 per session
    • Off-campus non-academic units: NT$15,000 per session
  • Each session lasts three hours (anything less is counted as three hours)
  • Note: Academic units refer to colleges and universities.
Instrument usage regulations:
  1. This instrument is designed for general tissue or cell samples. If you intend to experiment with other infectious samples, please discuss it with the technical operator first.
  2. Wearing gloves while operating computers is strictly prohibited.
  3. The standard operating procedures must be followed while using the instrument. Please make sure to comply with the instrument usage regulations. Failure to follow the regulations three times will suspend the right to use.
  4. Please thoroughly clean the instrument and operating surface after use. Fill out the instrument usage registration form and notify the instrument administrator to conduct an inspection.
  5. If the instrument malfunctions, please inform the technical personnel immediately. Do not attempt to disassemble or assemble the instrument yourself. Inform the technical operator or instrument manager of the instrument failure immediately. You must bear the related repair costs if the instrument is damaged for personal reasons.
  6. To access image-related data, bring your own DVD disc. Saving files on the computer and USB access are strictly prohibited. Violators will have their right to use it suspended for one month.
  7. If you publish papers in journals using this equipment, you can offset the one-time usage fee.
Instrument Management 
  1. The instrument is managed by full-time faculty members with experience operating it in the College of Life Science. Relevant personnel, funding, and usage issues are discussed with full-time faculty members with experience operating the instrument from the College of Life Science.
  2. Personnel, funding, and charging standards must be submitted to the Department's Resource Committee for resolution and implemented after approval by the Department Affairs Meeting.
  3. At least one or two seminars are held annually for education and training. If necessary, various training fees may be charged.
  4. The instrument has an assistant for equipment management and maintaining the surrounding environment. Depending on usage conditions, it will be decided whether to increase manpower.
  5. The instrument has several instrument seed instructors. They are postgraduate students with certification and operating licenses (certified and issued by the instrument manufacturer) who can lead and assist users in operating the instrument. In principle, seed instructors can be paid with a work-study allowance. However, no salary will be paid if the seed instructor leads their own laboratory users to operate the instruments.
Eligibility:
  1. Certification is required to use this instrument.
  2. Users are required to complete the "Principle of Temperature-Controlled Quantitative Bioprinting System" and "Operation of Temperature-Controlled Quantitative Bioprinting System" courses. After passing the certification, users will obtain usage rights.
Note: Educational training seminars for this instrument are held at least once or twice a year. Additional training may incur production costs.

Reservation:
  1. Reservations must be registered and submitted at least one week before the intended use date.
  2. After confirmation of the reservation, if you are unable to use the instrument, you must notify the cancellation at least three days before the scheduled use. If you fail to cancel your reservation at least three days in advance, it will be considered as if you used the reserved time slot, and you will still be responsible for payment.
     
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