Fluorescence Microscopes
中文- Update Date：2024-07-23
- Units：Instrumentation Resource Center
[YangMing] Slide Scanner - Zeiss Axioscan 7
- High-performance Slide Scanner for Fluorescence and Bright Field
- Instrument Location: Room 438, 4th Floor, Biomedical Engineering Building
- Person in charge: Yangming Campus Image Core Facility Specialist
- Ms. Pei-Jun Chen pjchen3@nycu.edu.tw
- Ms. Yung-yu Lu yungyu@nycu.edu.tw
- Term of use: Certification
- Only those who have passed the certification test after the instruction and have operated the device more than 5 times during office hours will be authorized to make a reservation.
- Charges：NT$300 per hour for NYCU users. Please refer to the charging standard for off-campus use
- Reservation >>> Instrument Reservation System
Microscope:
ZEISS Axioscan 7 Research Grade Fluorescent Slide Scanner (12-slice)
Objectives:
ZEISS Axioscan 7 Research Grade Fluorescent Slide Scanner (12-slice)
Objectives:
- 5X/0.25
- 10X/0.45
- 20X/0.5, 20X/0.8
- 40X/0.95
Fluorescence Illumination System:
Colibri 7 seven-channel LED light source: 385nm(UV), 430nm(V), 475nm(B), 555nm(G), 590nm(Y), 630nm(R), 735nm(Fr)
Observation Methods:
- Transmitted light：Bright Field, phase contrast (PH), and differential interference contrast (DIC) image
- Fluorescence：five sets of fluorescent filters
| Observation Method | Excitation | Emission |
|---|---|---|
| DAPI | UV (385nm) | EM 425/30 |
| GFP | Blue (475nm) | EM 514/30 |
| DsRed/mCherry | Green (555nm) / Yellow (590nm) | EM 592/25 |
| Cy5 | Red (630nm) | EM 681/45 |
| Cy7 | Far Red (735nm) | EM 788/38 |
Camera:
This machine is a dual image system, which can capture color images or moncrhome grayscale images
- Color CMOS：Axiocam 705 color, 5 million pixels
- Monochrome CMOS：Axiocam 712 mono, 12 million pixels
Slide size & Mounting Frames:
- Accommodates standard slides (26 x 76mm), wide slides (52 x 76 mm)
- Comes with three standard slide holders and one wide slide holder.
- Three slide holders can be placed simultaneously and accommodate up to 12 standard slides, each with individual acquisition conditions and settings.
Features:
- Automatic scan area detection
- Auto focus
- Batch scanning of transmitted light and fluorescent slides
- Automatic stitching
- Z-stack and Extended Depth of Field image
Software:
- ZEISS ZEN Blue：Download ZEISS Microscopy Installer, register, and follow the directions to download ZEN Lite for free.
- Offline workstation is available for image processing and analysis
- ZEN Module Intellesis + Image Analysis：Using machine learning methods (pixel classification and neural network) for image segmentation, as an alternative to traditional segmentation that is difficult to segment images
- Bright field sample observation: HE staining, silver staining, etc.
- Fluorescent immunostaining samples
- Support 4D image capture (XYZ λ)
- Automatic scan detection/auto focus/auto stitching
- Batch scanning: up to 12 standard slides with different acquisition parameters and settings can be placed at once
- Z-stack: motorized Z-axis scanning and Extended Depth of Field image processing
- Simple manual measurement or semi-automatic measurement after image segmentation with machine learning
*Users must be certified.
Users should pass the certification test and operate the system more than 5 times (including) during office hours (8:30-16:30) before they are authorized to reserve online, and can use the system according to the regulations. Each person can make 4 reservations within two weeks, and each reservation can be made for 4 hours.
Users should pass the certification test and operate the system more than 5 times (including) during office hours (8:30-16:30) before they are authorized to reserve online, and can use the system according to the regulations. Each person can make 4 reservations within two weeks, and each reservation can be made for 4 hours.
- Reservation regulations:
- Please go to the "Instrument Reservation System" to make a reservation before use. The person who makes the reservation must be the actual user.
- After use, please register in the record book. The start time is the reservation time, and the end time is based on the end time of use. You cannot delay the start time or occupy other people's time slots.
- Reservations cannot be canceled on the same day. Reservations made more than half an hour after the scheduled time are considered abandoned.
- No same-day reservations are accepted. Please confirm your reservation by 16:30 of the previous workday at the latest; late changes violate the rules and will not be accepted.
- Please carefully assess the time required for scanning and reserve enough time for imaging to not affect the next user.
- Sample requirements:
- Sample Size: (Please inquire with the administrator whether the slides are of compatible sizes on the first time use.)
- Standard slide 76mm*26mm
- Wide slide 76mm*52mm
- It is only suitable for fixed slide samples, and the slide must be completely sealed and cleaned before loading.
- Please be sure to clean your slides before loading.
- Always use the mounting aid when loading or removing slides from the mounting frames.
- Please ask the Instrument Center Administrator for assistance if you have any other needs or special usage requirements.
- Sample Size: (Please inquire with the administrator whether the slides are of compatible sizes on the first time use.)
- Monday to Friday all day00:00-24:00 Only Certified users can make reservations
| Fluorescence Microscope | Slide scanner | Upright type | Inverted type For living cells |
On Confocal Microscope For living cells |
|---|---|---|---|---|
| Model | (Z) Axioscan 7 | (L) DM6000B (O) BX61 (O) BX63 |
(O) IX83 | (Z) LSM880 (Z) LSM900 |
| Fees for NYCU faculty and students | 300 | 0 | 100 | 300 |
| Fees for cooperative educational units | 450 | 50 | 150 | 450 |
| Fees for other unit | 600 | 100 | 200 | 600 |
| Remarks |
|
Users must complete confocal microscope certification to use. | ||
If you have the following qualifications, you will be charged the same fee as NYCU faculty and students:
- Branch school
- Joint-appointed faculty
- Affiliated hospitals
- Projects in cooperation with and under the control of the University.
If you have the following qualifications, the fee will be the same as that of a cooperative educational unit:
- Adjunct faculty
- Teaching hospitals (Veterans General Hospital, Taipei City Hospital, Far Eastern Memorial Hospital, etc.)
- University System of Taiwan (UST)
- Companies participate in the academia-industry cooperation center of NYCU
Instruments are charged per half hour, and the billing will be counted at the end of each odd-numbered month. Please bring the bill to the Cashier's Section of the Administration Building within two weeks at the latest. The service will be stopped if the number of overdue payments reaches (and includes) two times.
- No USB flash drive is allowed. Please use FTP to transfer files or bring your blank CD-ROM to burn data. Due to the limited space of the computer's hard disk, the administrator will delete the data from time to time to facilitate the normal operation of the instrument. We are not responsible for data storage.
- Without the consent of the administrator of the Center, you are not allowed to add or remove any parts, alter the system settings, or install software on the Center's public equipment. If any experiment requires any test, the administrator's consent must be sought first and accompanied by the engineer of the original manufacturer to carry out the testing without damaging the normal operation of the equipment, and only after the passing of the test can it be operated and used by the personnel approved by the administrator of the Center under the condition of the administrator's permission.
- According to the administrator's judgment, the violator is warned for the first time, canceled the right to use for one month for the second time, and canceled the right to use permanently for the third time; if the violator violates the above specification, resulting in the damage of the instrument, the violator shall pay compensation to repair the instrument and stop the right to use for one month. The violator is prohibited from using the instrument permanently.
- Axioscan-7 User Manual
- Certification-form-FL_2022
- How-to-choose-FL-microscope
Related Image(s)：
- Contact： [ ext. 65980]
