Real-time PCR
中文- Update Date：2025-04-30
- Units：Instrumentation Resource Center
[YangMing] CFX Opus PCR System
- Brand/Model: BIO-RAD CFX Opus
- Instrument Location: Yang-Ming Campus
- Room B02, Shou-Ren Building (S machine)
- Term of Use: Reservation required
- Free of charge for NYCU users. Off-campus users, please contact the center before using it.
- Reservation >>> Instrument Reservation System
- Contact Center Staff：
- Extension：62382
- Email：ycirc@nycu.edu.tw
Features:
- Detects 5 fluorescence types simultaneously and utilizes a sweeping, independent light collection design to avoid cross-talk between samples. Patented optical design - users can use a specified spectra range for "single channel excitation" and detect fluorescence signals in each reaction well.
- Fluorescence excitation ranges from 450-730 nm. Suitable dyes include Cy5, Fluorescein (FAM), SYBR Green, HEX, Texas Red, ROX, Cal gold 540, Cal red 610, Quasar 670, and Quasar 705.
- Experimental Applications: It can be set to capture two (or more) fluorescent time points in a heat cycle for different response modes, including Hybridization probe (Molecular Beacons, FRET pairs), DNA binding dye (SYBR Green I), Hydrolysis Probes, MethylTagMan, Scorpion, and Differential Scanning Fluorimetry (DSF)。
- Dynamic temperature gradient function: This feature allows for gradient temperature control of '8 sections (A - H zones)' simultaneously. For instance, it can be used to efficiently and quickly optimize the experimental reaction and find the ideal experimental conditions for PCR amplification or DNA sequencing. Gradient temperature can be set to 30~100℃, and the range of temperature distribution above and below the gradient is 1~24℃.
- Not only does it have higher sensitivity (1 copy of target sequence inhuman genome DNA), but it also avoids fluorescent cross-talks between reactions and can eliminate the influence of optical path difference simultaneously (no need to use additional fluorescent ROX correction).
- Software analysis applications include:
- Absolute quantization
- Relative quantization
- Multiple plates combination
- Multiple reference genes
- Efficiency estimation
- Genotyping (Allelic discrimination, SNP)
- Endpoint analysis
- Automated statistics analysis: through the quick setting of p-value, it is possible to easily complete the "t-test" and "One Way ANOVA" analysis with one click.
- Please make an online reservation before use. (For detailed instructions, please refer to the reservation rules)
- Users are responsible for promptly filling in the registration book and retrieving their samples after the experiment. In case of any damage to the sample, the center will not be responsible for it.
- Users are not allowed to perform the following operations on the computers of the center:
- Change programs or parameters or other people's files.
- Externally connect any peripheral devices.
- Copy or install any program on the center's computer (the programs on the center's computer are all original and have copyright).
- Violators will be prohibited from using it forever. Violators will be punished according to the school rules for any damage caused.
- If you have any questions when using it, please ask the instrument center technicians.
- Restricted to use 0.1ml PCR 96-well plate or tube (only Low Profile tube or plate can be used.) If you use other specifications and cause damage to the machine, you will be liable for repair and compensation!
- The person making the reservation must be the actual user and cannot book on behalf of someone else.
- The earliest available appointment is two weeks in advance. For example, if today is Tuesday, the earliest available reservation would be for next Monday.
- Each user can reserve a maximum of two sessions at a time, with a required interval between the sessions. Additionally, each slot cannot exceed 3 hours.
- If you are more than 30 minutes late for the reserved period, it will be considered as giving up the slot, and others can use it at any time.
- The access or return time of accessories should not affect the use of others.
- Please take your personal belongings with you and do not leave any items behind (especially samples).
Note:
Cancellations must be made as soon as possible to avoid causing inconvenience to others. Frequent no-shows without prior cancellation will result in strict limitations on future reservations after investigation.
- Analysis software: CFX Maestro 2.0 software (Recommended operating system: Win10 or above)
- User Manual (Eng. version)
- Instructional Video - Principles
- Instructional Video - operation instructions
- Education Training - Principle explanation (Eng. version)
- Education Training - Instructions (Eng. version)
Related Image(s)：
