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Fluorescence Microscopes

exchangealt 中文
  • Update Date：2025-01-15
  • Units：Instrumentation Resource Center
[YangMing] Upright Microscope - Olympus BX63
Upright Motorized Fluorescence Microscope Olympus BX63
  • Motorized Upright Fluorescence Microscope
  • Instrument Location: Yang-Ming Campus
    • Room B03, Floor B1, Shou-ren Building
  • Person in charge: YM Campus Imaging Core Facility
  • Term of use: Certification 
    • Only those who have passed the certification test after the instruction and have operated the device more than 5 times during office hours will be authorized to make a reservation.
    • Free of charge for on-campus use. Please refer to the charging standard for off-campus use.
  • Reservation >>> Instrument Reservation System
Microscope System:
  • Olympus BX-63 Upright Electronic Control Microscope
  • Color LCD touch panel
  • BX3-SSU ultrasonic motorized stage (can hold two standard slides)
Objectives:
  • 1.25X, 4X, 10X, 20X, 40X
  • 100X (Oil)
Fluorescence Illumination System:
  • X-cite TURBO LED
Observation Methods:
  • Transmitted light: Bright Field (BF) and Differential Interference Contrast (DIC)
  • Fluorescence: Fluorescence filter with ultraviolet light excitation, blue light excitation, yellow-green light excitation, and near-infrared light BX63 filter sets) (Note 1)BX63 filter sets
Camera:
  • DP72 High-sensitivity cooled color CCD
Software:
  • cellSens V2.3
  • Including Dimension and Multi Position advanced modules
  • Deconvolution: 2D, Nearest Neighbor and Wiener
Note 1. On 2023/9/19 Replaced new fluorescent light source and updated a fast fluorescent turntable 89402 ET-Multi LED set
Method LED Mirror Unit Excitation Filter Dichromatic Mirror Emission Filter
DAPI 385 89402 ET-Multi LED set 391/32 410-458 418-450
GFP 475 89402 ET-Multi LED set 479/33 497-540 505-530
TRITC 525 89402 ET-Multi LED set 554/24 570-615 577-610
Cy5 630 89402 ET-Multi LED set 638/31 655-730 663-725
FITC-long 475 U-FBW BP460-495 DM505 BA510IF
mCherry 575 U-FYW BP545-585 DM595 BA600IF
  • Bright field sample observation: HE staining, silver staining, etc.
  • Fluorescent immunostaining samples
  • Support 5D image capture (XYZ λ T)
  • Motorized Multi-Channel Scanner
  • Multi Position: motorized multi-position or area scanning
  • Z-slices: motorized Z-axis scan and EFI (extended depth of field) processing
  • Simple manual measurement
*Users must be certified.
Users should pass the certification test and operate the system more than 5 times (including) during office hours (8:30-16:30) before they are authorized to reserve online, and can use the system according to the regulations. If the certified user does not use the device for one month, he/she must be certified again by the administrator.
 
  1. Reservation regulations:
    • Please go to the "Instrument Reservation System" to make a reservation before use. The person who makes the reservation must be the actual user.
    • After use, please register in the record book. The start time is the reservation time, and the end time is based on the end time of use. You cannot delay the start time or occupy other people's time slots.
    • Reservations cannot be canceled on the same day. Reservations made more than half an hour after the scheduled time are considered abandoned.
    • No same-day reservations are accepted. Please confirm your reservation by 16:30 of the previous workday at the latest; late changes violate the rules and will not be accepted.
    • Each person can make 4 reservations within two weeks, and each reservation can be made for 4 hours.
  2. Sample requirements:
    • Fixed slide samples must be completely sealed before loading.
    • Please be sure to clean slides before loading.
    • Please ask the Instrument Center Administrator for assistance if you have any other needs or special usage requirements.
  • Monday to Friday
    • 00:00-24:00 Restricted to Certified Users only 
  • weekend
    • 00:00-24:00 Restricted to Certified Users only
Instrument charges (NTD per hour)
Fluorescence Microscope Slide scanner Upright type Inverted type
For living cells		 On Confocal Microscope
For living cells 
Model (Z) Axioscan 7 (L) DM6000B
(O) BX61
(O) BX63		 (O) IX83 (Z) LSM880 with Airyscan
(Z) LSM900 with Airyscan2
Fees for NYCU faculty and students 300 0 100 300
Fees for cooperative educational units 450 50 150 450
Fees for other unit 600 100 200 600
Remarks
  1. Abbreviation: (L) Leica, (O) Olympus, (Z) Zeiss
  2. Number of Reservations: Each person can make four reservations per device within a two-week period, with a maximum of four hours per reservation. Reservations can be made consecutively on all devices.
  3. Besides the imaging core facility education and training organized by the center, it is recommended that you bring your samples for scheduled teaching and certification exams (experimental data can be taken away), and the charges will be the same as for general use.
  4. The above table shows the hourly usage charge. Charges are calculated on a half-hour basis.
 Users must complete confocal microscope certification to use.
If you have the following qualifications, you will be charged the same fee as NYCU faculty and students:
  • Branch school
  • Joint-appointed faculty 
  • Affiliated hospitals 
  • Projects in cooperation with and under the control of the University.
If you have the following qualifications, the fee will be the same as that of a cooperative educational unit:
  • Adjunct faculty
  • Teaching hospitals (Veterans General Hospital, Taipei City Hospital, Far Eastern Memorial Hospital, etc.)
  • University System of Taiwan (UST)
  • Companies participate in the academia-industry cooperation center of NYCU
Instruments are charged per half hour, and the billing will be counted at the end of each odd-numbered month. Please bring the bill to the Cashier's Section of the Administration Building within two weeks at the latest. The service will be stopped if the number of overdue payments reaches (and includes) two times.
  1. No USB flash drive is allowed. Please use FTP to transfer files or bring your blank CD-ROM to burn data. Due to the limited space of the computer's hard disk, the administrator will delete the data from time to time to facilitate the normal operation of the instrument. We are not responsible for data storage.
  2. Without the consent of the administrator of the Center, you are not allowed to add or remove any parts, alter the system settings, or install software on the Center's public equipment. If any experiment requires any test, the administrator's consent must be sought first and accompanied by the engineer of the original manufacturer to carry out the testing without damaging the normal operation of the equipment, and only after the passing of the test can it be operated and used by the personnel approved by the administrator of the Center under the condition of the administrator's permission.
  3. According to the administrator's judgment, the violator is warned for the first time, canceled the right to use for one month for the second time, and canceled the right to use permanently for the third time; if the violator violates the above specification, resulting in the damage of the instrument, the violator shall pay compensation to repair the instrument and stop the right to use for one month. The violator is prohibited from using the instrument permanently.
  • How-to-choose-FL-microscope
  • Certification-form-FL_2022
  • User Manual (English version)
  • CellSens Manual：English version from Olympus
Instrument List of YM Campus Imaging Core of YM Campus Other Fluorescence Microscopes
Related Image(s)：
  • Contact： [ ext. 66185/65980]
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