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Fluorescence Microscopes

exchangealt 中文
  • Update Date：2025-01-15
  • Units：Instrumentation Resource Center
[YangMing] Inverted Microscope with Incubation System - Olympus IX83
Inverted Motorized Fluorescence Microscope Olympus IX83
  • Motorized Inverted Fluorescence Microscope with Incubation System
  • Instrument Location: Yang-Ming Campus
    • Room 617, 6th Floor, Library, Information & Research Building
  • Person in charge: YM Campus Imaging Core Facility 
  • Term of use: Users are required to be certified to use.
    • Only those who have passed the certification test after the instruction and have operated the device more than 5 times during office hours will be authorized to make a reservation.
  • Charges: NT $100 per hour for NYCU users. Please refer to the charging standard for off-campus use.
  • Reservation >>> Instrument Reservation System
Microscope:
  • Inverted Motorized microscope Olympus IX83
  • Color LCD touch panel
  • Ultrasonic electric stage
Objectives:
  • 2X
  • 4X PH
  • 10X PH
  • 20X PH
  • 40X DIC (LWD plan semi apochromat LUCPLFLN 40X)
  • 60X Silicone/DIC silicone oil lens (please ask the administrator if you need to use it)
Fluorescence Illumination System:
Observation Methods:
  • Transmitted Light: Bright Field (BF), Phase Contrast (PH) and Differential Interference Contrast (DIC)
  • Fluorescence: There are three sets of eight channels of fluorescence filters (Click here to download)
Observation and Filter Configurations
Observation Methods Excitation Filter Dichromatic Mirror Emission Filter
DAPI 387/11 410 440/40
GFP 485/20 504 525/30
TRITC 560/20 582 607/36
Cy5 650/13 669 684/24
CFP 427/10 440 472/30
YFP 504/12 520 542/27
mCherry 575/20 595 645/90
FITC-long 480/40 505 510IF

Camera:
It is equipped with a dual-image system that can capture color images or black-and-white grayscale images
  • Olympus SC180: Color CMOS
  • HAMAMATSU ORCA-Flash4.0 V3: Monochrome sCMOS
Live-cell Incubation:
Tokai Hit live cell stage incubator includes a temperature-controlled black box cover and a small stage-top incubator. Different sample carriers can be replaced according to sample types (35mm dish, standard well plate, chamber slide, chambered cover glass, etc.)
Stage-top incubation system
The picture above shows a stage-top incubator, installed on the ultrasonic electric stage. The CO2 concentration control range is 5.0 to 20%, and the temperature can be controlled from 30 to 40℃ at room temperature of 25±2℃.

Software:
  • cellSens: including Dimension, Multi-Position, Well plate navigator, CI Deconvolution advanced module Deconvolution: 2D, Nearest Neighbor and Wiener
  • cellSens Dimension image capture analysis
  • Offline computer workstation is available for image processing and analysis
  • Time-lapse live-cell imaging (with automatic focus maintenance system, ZDC2)
  • Bright field sample observation: HE staining, silver staining, etc.
  • Fluorescence immunostaining samples
  • 5D image capture (X Y Z λ T)
  • Multi-Channel imaging
  • Multi-Position or multi-area scanning
  • Well navigator: automatic multi- position imaging of 4, 8, 12, 24, 48, 96-well plate, Chamber slice, etc. 
  • Z-slices: motorized Z-axis scanning and extended depth of field (EFI) processing
  • Simple manual measurements
*Users must be certified.
Users should pass the certification test and operate the system more than 5 times (including) during office hours (8:30-16:30) before they are authorized to reserve online, and can use the system according to the regulations. If the certified user does not use the device for one month, he/she must be certified again by the administrator.
 
  1. Reservation regulations:
    • Please go to the "Instrument Reservation System" to make a reservation before use. The person who makes the reservation must be the actual user.
    • After use, please register in the record book. The start time is the reservation time, and the end time is based on the end time of use. You cannot delay the start time or occupy other people's time slots.
    • Reservations cannot be canceled on the same day. Reservations made more than half an hour after the scheduled time are considered abandoned.
    • No same-day reservations are accepted. Please confirm your reservation by 16:30 of the previous workday at the latest; late changes violate the rules and will not be accepted.
    • Each person can make 4 reservations within two weeks, and each reservation can be made for 4 hours.
  2. Sample requirements:
    • Sample Carrier (Please ask the administrator if the sample is compatible for the first time)
      1. Standard multiwell plate: 6/12/24/96-well cell culture plate
        The following sample specifications can be applied after replacing the inner carrier:
      2. Universal holder: chamber slide/slide glass/chambered cover glass
      3. Culture dish: 35/50/60mm petri dish
      4. Slide: standard 76mm*26mm
Sample stage
  • For long-term live cell imaging, please inform the administrator about the thickness of the sample plate to set the appropriate parameters for auto-focus maintenance.
  • If you have other requirements or special needs, please ask the administrator to provide relevant consultation services.
  • Weekdays (Monday to Friday) 00:00-24:00; Online reservation for certified users only
  • Weekends (Saturaday to Sunday) 00:00-24:00; Priority is given to the demand of long-time live-cell imaging.
If you need long-term time-lapse live-cell imaging, please contact the instrument administrator for a reservation.
Instrument charges (NTD per hour)
Fluorescence Microscope Slide scanner Upright type Inverted type
For living cells		 On Confocal Microscope
For living cells 
Model (Z) Axioscan 7 (L) DM6000B
(O) BX61
(O) BX63		 (O) IX83 (Z) LSM880 with Airyscan
(Z) LSM900 with Airyscan2
Fees for NYCU faculty and students 300 0 100 300
Fees for cooperative educational units 450 50 150 450
Fees for other unit 600 100 200 600
Remarks
  1. Abbreviation: (L) Leica, (O) Olympus, (Z) Zeiss
  2. Number of Reservations: Each person can make four reservations per device within a two-week period, with a maximum of four hours per reservation. Reservations can be made consecutively on all devices.
  3. Besides the imaging core facility education and training organized by the center, it is recommended that you bring your samples for scheduled teaching and certification exams (experimental data can be taken away), and the charges will be the same as for general use.
  4. The above table shows the hourly usage charge. Charges are calculated on a half-hour basis.
 Users must complete confocal microscope certification to use.
If you have the following qualifications, you will be charged the same fee as NYCU faculty and students:
  • Branch school
  • Joint-appointed faculty 
  • Affiliated hospitals 
  • Projects in cooperation with and under the control of the University.
If you have the following qualifications, the fee will be the same as that of a cooperative educational unit:
  • Adjunct faculty
  • Teaching hospitals (Veterans General Hospital, Taipei City Hospital, Far Eastern Memorial Hospital, etc.)
  • University System of Taiwan (UST)
  • Companies participate in the academia-industry cooperation center of NYCU
Instruments are charged per half hour, and the billing will be counted at the end of each odd-numbered month. Please bring the bill to the Cashier's Section of the Administration Building within two weeks at the latest. The service will be stopped if the number of overdue payments reaches (and includes) two times.
  1. No USB flash drive is allowed. Please use FTP to transfer files or bring your blank CD-ROM to burn data. Due to the limited space of the computer's hard disk, the administrator will delete the data from time to time to facilitate the normal operation of the instrument. We are not responsible for data storage.
  2. Without the consent of the administrator of the Center, you are not allowed to add or remove any parts, alter the system settings, or install software on the Center's public equipment. If any experiment requires any test, the administrator's consent must be sought first and accompanied by the engineer of the original manufacturer to carry out the testing without damaging the normal operation of the equipment, and only after the passing of the test can it be operated and used by the personnel approved by the administrator of the Center under the condition of the administrator's permission.
  3. According to the administrator's judgment, the violator is warned for the first time, canceled the right to use for one month for the second time, and canceled the right to use permanently for the third time; if the violator violates the above specification, resulting in the damage of the instrument, the violator shall pay compensation to repair the instrument and stop the right to use for one month. The violator is prohibited from using the instrument permanently.
  • How-to-choose-FL-microscope
  • Certification-form-FL_2022
  • Instrument Operation Manual (Chinese version)
Instrument List of YM Campus Imaging Core of YM Campus Other Fluorescence Microscopes
Related Image(s)：
  • Contact： [ ext. 66185]
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